Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women

Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women

The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family mediates parasite sequestration in small capillaries through tissue-specific cytoadherence. The best characterized of these proteins is VAR2CSA, which is expressed on the surface of infected erythrocytes that bind to chondroiti...

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Main Authors: Travassos, Mark A., Coulibaly, Drissa, Bailey, Jason A., Niangaly, Amadou, Adams, Matthew, Nyunt, Myaing M., Ouattara, Amed, Lyke, Kirsten E., Laurens, Matthew B., Pablo, Jozelyn, Jasinskas, Algis, Nakajima, Rie, Berry, Andrea A., Takala-Harrison, Shannon, Kone, Abdoulaye K., Kouriba, Bourema, Rowe, J. Alexandra, Doumbo, Ogobara K., Thera, Mahamadou A., Laufer, Miriam K., Felgner, Philip L., Plowe, Christopher V.
Format: Online
Language:English
Published: The American Society of Tropical Medicine and Hygiene 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458824/
id pubmed-4458824
recordtype oai_dc
spelling pubmed-44588242015-06-11 Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women Travassos, Mark A. Coulibaly, Drissa Bailey, Jason A. Niangaly, Amadou Adams, Matthew Nyunt, Myaing M. Ouattara, Amed Lyke, Kirsten E. Laurens, Matthew B. Pablo, Jozelyn Jasinskas, Algis Nakajima, Rie Berry, Andrea A. Takala-Harrison, Shannon Kone, Abdoulaye K. Kouriba, Bourema Rowe, J. Alexandra Doumbo, Ogobara K. Thera, Mahamadou A. Laufer, Miriam K. Felgner, Philip L. Plowe, Christopher V. Articles The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family mediates parasite sequestration in small capillaries through tissue-specific cytoadherence. The best characterized of these proteins is VAR2CSA, which is expressed on the surface of infected erythrocytes that bind to chondroitin sulfate in the placental matrix. Antibodies to VAR2CSA prevent placental cytoadherence and protect against placental malaria. The size and complexity of the VAR2CSA protein pose challenges for vaccine development, but smaller constitutive domains may be suitable for subunit vaccine development. A protein microarray was printed to include five overlapping fragments of the 3D7 VAR2CSA extracellular region. Malian women with a history of at least one pregnancy had antibody recognition of four of these fragments and had stronger reactivity against the two distal fragments than did nulliparous women, children, and men from Mali, suggesting that the C-terminal extracellular VAR2CSA domains are a potential focus of protective immunity. With carefully chosen sera from longitudinal studies of pregnant women, this approach has the potential to identify seroreactive VAR2CSA domains associated with protective immunity against pregnancy-associated malaria. The American Society of Tropical Medicine and Hygiene 2015-06-03 /pmc/articles/PMC4458824/ /pubmed/25918203 http://dx.doi.org/10.4269/ajtmh.14-0524 Text en ©The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Travassos, Mark A.
Coulibaly, Drissa
Bailey, Jason A.
Niangaly, Amadou
Adams, Matthew
Nyunt, Myaing M.
Ouattara, Amed
Lyke, Kirsten E.
Laurens, Matthew B.
Pablo, Jozelyn
Jasinskas, Algis
Nakajima, Rie
Berry, Andrea A.
Takala-Harrison, Shannon
Kone, Abdoulaye K.
Kouriba, Bourema
Rowe, J. Alexandra
Doumbo, Ogobara K.
Thera, Mahamadou A.
Laufer, Miriam K.
Felgner, Philip L.
Plowe, Christopher V.
spellingShingle Travassos, Mark A.
Coulibaly, Drissa
Bailey, Jason A.
Niangaly, Amadou
Adams, Matthew
Nyunt, Myaing M.
Ouattara, Amed
Lyke, Kirsten E.
Laurens, Matthew B.
Pablo, Jozelyn
Jasinskas, Algis
Nakajima, Rie
Berry, Andrea A.
Takala-Harrison, Shannon
Kone, Abdoulaye K.
Kouriba, Bourema
Rowe, J. Alexandra
Doumbo, Ogobara K.
Thera, Mahamadou A.
Laufer, Miriam K.
Felgner, Philip L.
Plowe, Christopher V.
Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
author_facet Travassos, Mark A.
Coulibaly, Drissa
Bailey, Jason A.
Niangaly, Amadou
Adams, Matthew
Nyunt, Myaing M.
Ouattara, Amed
Lyke, Kirsten E.
Laurens, Matthew B.
Pablo, Jozelyn
Jasinskas, Algis
Nakajima, Rie
Berry, Andrea A.
Takala-Harrison, Shannon
Kone, Abdoulaye K.
Kouriba, Bourema
Rowe, J. Alexandra
Doumbo, Ogobara K.
Thera, Mahamadou A.
Laufer, Miriam K.
Felgner, Philip L.
Plowe, Christopher V.
author_sort Travassos, Mark A.
title Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
title_short Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
title_full Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
title_fullStr Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
title_full_unstemmed Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women
title_sort differential recognition of terminal extracellular plasmodium falciparum var2csa domains by sera from multigravid, malaria-exposed malian women
description The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family mediates parasite sequestration in small capillaries through tissue-specific cytoadherence. The best characterized of these proteins is VAR2CSA, which is expressed on the surface of infected erythrocytes that bind to chondroitin sulfate in the placental matrix. Antibodies to VAR2CSA prevent placental cytoadherence and protect against placental malaria. The size and complexity of the VAR2CSA protein pose challenges for vaccine development, but smaller constitutive domains may be suitable for subunit vaccine development. A protein microarray was printed to include five overlapping fragments of the 3D7 VAR2CSA extracellular region. Malian women with a history of at least one pregnancy had antibody recognition of four of these fragments and had stronger reactivity against the two distal fragments than did nulliparous women, children, and men from Mali, suggesting that the C-terminal extracellular VAR2CSA domains are a potential focus of protective immunity. With carefully chosen sera from longitudinal studies of pregnant women, this approach has the potential to identify seroreactive VAR2CSA domains associated with protective immunity against pregnancy-associated malaria.
publisher The American Society of Tropical Medicine and Hygiene
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458824/
_version_ 1613232711505281024

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