Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation

Estrogen plays an important role in inhibiting osteoclast differentiation and protecting against bone loss from osteoporosis, especially in postmenopausal women. However, the precise mechanisms underlying the effect of estrogen on osteoclasts are not well known. In the present study, we performed pr...

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Main Authors:	Xiong, Qi, Tang, Peifu, Gao, Yanpan, Zhang, Lihai, Ge, Wei
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450280/

id	pubmed-4450280
recordtype	oai_dc
spelling	pubmed-44502802015-06-28 Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation Xiong, Qi Tang, Peifu Gao, Yanpan Zhang, Lihai Ge, Wei Research Article Estrogen plays an important role in inhibiting osteoclast differentiation and protecting against bone loss from osteoporosis, especially in postmenopausal women. However, the precise mechanisms underlying the effect of estrogen on osteoclasts are not well known. In the present study, we performed proteomics analysis and bioinformatics analysis to comprehensively compare the differential expression of proteins in receptor activator of nuclear factor-κB ligand RANKL-induced osteoclasts in the presence and absence of estrogen. We identified 6403 proteins, of which 124 were upregulated and 231 were downregulated by estrogen. Bioinformatics analysis showed that estrogen treatment interfered with 77 intracellular pathways, including both confirmed canonical and unconfirmed pathways of osteoclast formation. Our findings validate the inhibitory effect of estrogen on osteoclasts via the promotion of apoptosis and suppression of differentiation and polarization and suggest that estrogen might inhibit osteoclast formation via other pathways, which requires further investigation and verification. Hindawi Publishing Corporation 2015 2015-05-18 /pmc/articles/PMC4450280/ /pubmed/26120583 http://dx.doi.org/10.1155/2015/596789 Text en Copyright © 2015 Qi Xiong et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xiong, Qi Tang, Peifu Gao, Yanpan Zhang, Lihai Ge, Wei
spellingShingle	Xiong, Qi Tang, Peifu Gao, Yanpan Zhang, Lihai Ge, Wei Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
author_facet	Xiong, Qi Tang, Peifu Gao, Yanpan Zhang, Lihai Ge, Wei
author_sort	Xiong, Qi
title	Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
title_short	Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
title_full	Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
title_fullStr	Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
title_full_unstemmed	Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
title_sort	proteomic analysis of estrogen-mediated signal transduction in osteoclasts formation
description	Estrogen plays an important role in inhibiting osteoclast differentiation and protecting against bone loss from osteoporosis, especially in postmenopausal women. However, the precise mechanisms underlying the effect of estrogen on osteoclasts are not well known. In the present study, we performed proteomics analysis and bioinformatics analysis to comprehensively compare the differential expression of proteins in receptor activator of nuclear factor-κB ligand RANKL-induced osteoclasts in the presence and absence of estrogen. We identified 6403 proteins, of which 124 were upregulated and 231 were downregulated by estrogen. Bioinformatics analysis showed that estrogen treatment interfered with 77 intracellular pathways, including both confirmed canonical and unconfirmed pathways of osteoclast formation. Our findings validate the inhibitory effect of estrogen on osteoclasts via the promotion of apoptosis and suppression of differentiation and polarization and suggest that estrogen might inhibit osteoclast formation via other pathways, which requires further investigation and verification.
publisher	Hindawi Publishing Corporation
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450280/
_version_	1613229923402514432

Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation

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