Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
Glial scar formation is a major obstacle to regeneration after spinal cord injury. Moreover, it has been shown that the astrocytic response to injury differs between species. Gekko japonicas is a type of reptile and it shows differential glial activation compared to that of rats. The purpose of the...
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Public Library of Science
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447339/ |
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pubmed-44473392015-06-09 Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro Gu, Yun Yang, Jian Chen, Haijiao Li, Jing Xu, Man Hua, Juan Yao, Jian Wang, Yongjun Liu, Yan Liu, Mei Research Article Glial scar formation is a major obstacle to regeneration after spinal cord injury. Moreover, it has been shown that the astrocytic response to injury differs between species. Gekko japonicas is a type of reptile and it shows differential glial activation compared to that of rats. The purpose of the present study was to compare the proliferation and migration of astrocytes in the spinal cords of geckos and rats after injury in vitro. Spinal cord homogenate stimulation and scratch wound models were used to induce astrocytic activation in adult and embryonic rats, as well as in adult geckos. Our results indicated that astrocytes from the adult rat were likely activated by mechanical stimulation, even though they showed lower proliferation abilities than the astrocytes from the gecko under normal conditions. Furthermore, a transcriptome analysis revealed that the differentially expressed genes in astrocytes from adult rats and those from geckos were enriched in pathways involved in proliferation and the response to stimuli. This implies that intrinsic discrepancies in gene expression patterns might contribute to the differential activation of astrocytes between species. Public Library of Science 2015-05-28 /pmc/articles/PMC4447339/ /pubmed/26020931 http://dx.doi.org/10.1371/journal.pone.0127663 Text en © 2015 Gu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Gu, Yun Yang, Jian Chen, Haijiao Li, Jing Xu, Man Hua, Juan Yao, Jian Wang, Yongjun Liu, Yan Liu, Mei |
| spellingShingle |
Gu, Yun Yang, Jian Chen, Haijiao Li, Jing Xu, Man Hua, Juan Yao, Jian Wang, Yongjun Liu, Yan Liu, Mei Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro |
| author_facet |
Gu, Yun Yang, Jian Chen, Haijiao Li, Jing Xu, Man Hua, Juan Yao, Jian Wang, Yongjun Liu, Yan Liu, Mei |
| author_sort |
Gu, Yun |
| title |
Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
|
| title_short |
Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
|
| title_full |
Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
|
| title_fullStr |
Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
|
| title_full_unstemmed |
Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro
|
| title_sort |
different astrocytic activation between adult gekko japonicus and rats during wound healing in vitro |
| description |
Glial scar formation is a major obstacle to regeneration after spinal cord injury. Moreover, it has been shown that the astrocytic response to injury differs between species. Gekko japonicas is a type of reptile and it shows differential glial activation compared to that of rats. The purpose of the present study was to compare the proliferation and migration of astrocytes in the spinal cords of geckos and rats after injury in vitro. Spinal cord homogenate stimulation and scratch wound models were used to induce astrocytic activation in adult and embryonic rats, as well as in adult geckos. Our results indicated that astrocytes from the adult rat were likely activated by mechanical stimulation, even though they showed lower proliferation abilities than the astrocytes from the gecko under normal conditions. Furthermore, a transcriptome analysis revealed that the differentially expressed genes in astrocytes from adult rats and those from geckos were enriched in pathways involved in proliferation and the response to stimuli. This implies that intrinsic discrepancies in gene expression patterns might contribute to the differential activation of astrocytes between species. |
| publisher |
Public Library of Science |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4447339/ |
| _version_ |
1613228911063203840 |