Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats

Total body irradiation (TBI) is part of the preconditioning regimen for allogeneic bone marrow transplantation (alloBMT) and the procedure is associated with treatment-related toxicity and delayed immune reconstitution. Natural killer (NK) cells develop and acquire functional competence in close int...

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Main Authors:	Nestvold, Janne M., Rolstad, Bent
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444966/

id	pubmed-4444966
recordtype	oai_dc
spelling	pubmed-44449662015-06-12 Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats Nestvold, Janne M. Rolstad, Bent Endocrinology Total body irradiation (TBI) is part of the preconditioning regimen for allogeneic bone marrow transplantation (alloBMT) and the procedure is associated with treatment-related toxicity and delayed immune reconstitution. Natural killer (NK) cells develop and acquire functional competence in close interaction with stromal bone marrow cells that are considered relatively radioresistant compared to the hematopoietic compartment. We thus undertook a study to assess the effect of TBI on the reconstitution of class I MHC-specific Ly49 NK cell receptors in a rat model of alloBMT. In rats subjected to TBI alone or followed by MHC-matched BMT, the irradiation conditioning induced a skewing of the Ly49 repertoire. Specifically, the activating Ly49s3bright subset exhibited increased frequency and receptor density which correlated with augmented alloreactivity relative to untreated control rats. Our results highlight the plasticity of NK cells and indicate that ionizing radiation (IR) affects the stromal compartment and as a consequence the maturation and functional properties of bone marrow-derived NK cells. These changes lasted throughout the 6 months observation period, showing that irradiation induces long term effects on the generation of the NK cell receptor repertoire. Frontiers Media S.A. 2015-05-27 /pmc/articles/PMC4444966/ /pubmed/26075203 http://dx.doi.org/10.3389/fcell.2015.00034 Text en Copyright © 2015 Nestvold and Rolstad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Nestvold, Janne M. Rolstad, Bent
spellingShingle	Nestvold, Janne M. Rolstad, Bent Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
author_facet	Nestvold, Janne M. Rolstad, Bent
author_sort	Nestvold, Janne M.
title	Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
title_short	Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
title_full	Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
title_fullStr	Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
title_full_unstemmed	Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats
title_sort	irradiation and bone marrow reconstitution affect the functional ly49 natural killer cell repertoire in rats
description	Total body irradiation (TBI) is part of the preconditioning regimen for allogeneic bone marrow transplantation (alloBMT) and the procedure is associated with treatment-related toxicity and delayed immune reconstitution. Natural killer (NK) cells develop and acquire functional competence in close interaction with stromal bone marrow cells that are considered relatively radioresistant compared to the hematopoietic compartment. We thus undertook a study to assess the effect of TBI on the reconstitution of class I MHC-specific Ly49 NK cell receptors in a rat model of alloBMT. In rats subjected to TBI alone or followed by MHC-matched BMT, the irradiation conditioning induced a skewing of the Ly49 repertoire. Specifically, the activating Ly49s3bright subset exhibited increased frequency and receptor density which correlated with augmented alloreactivity relative to untreated control rats. Our results highlight the plasticity of NK cells and indicate that ionizing radiation (IR) affects the stromal compartment and as a consequence the maturation and functional properties of bone marrow-derived NK cells. These changes lasted throughout the 6 months observation period, showing that irradiation induces long term effects on the generation of the NK cell receptor repertoire.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444966/
_version_	1613228001847148544

Irradiation and bone marrow reconstitution affect the functional Ly49 natural killer cell repertoire in rats

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