Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection

The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airb...

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Main Authors:	Sharma, Anurag, Clark, Elizabeth, McGlothlin, James D., Mittal, Suresh K.
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444837/

id	pubmed-4444837
recordtype	oai_dc
spelling	pubmed-44448372015-06-12 Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection Sharma, Anurag Clark, Elizabeth McGlothlin, James D. Mittal, Suresh K. Microbiology The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3) in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5 × 103 plaque-forming units (p.f.u.) [2 × 105 genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection. Frontiers Media S.A. 2015-05-27 /pmc/articles/PMC4444837/ /pubmed/26074900 http://dx.doi.org/10.3389/fmicb.2015.00512 Text en Copyright © 2015 Sharma, Clark, McGlothlin and Mittal. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sharma, Anurag Clark, Elizabeth McGlothlin, James D. Mittal, Suresh K.
spellingShingle	Sharma, Anurag Clark, Elizabeth McGlothlin, James D. Mittal, Suresh K. Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
author_facet	Sharma, Anurag Clark, Elizabeth McGlothlin, James D. Mittal, Suresh K.
author_sort	Sharma, Anurag
title	Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
title_short	Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
title_full	Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
title_fullStr	Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
title_full_unstemmed	Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
title_sort	efficiency of airborne sample analysis platform (asap) bioaerosol sampler for pathogen detection
description	The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3) in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5 × 103 plaque-forming units (p.f.u.) [2 × 105 genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444837/
_version_	1613227971668082688

Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection

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