Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle

Curcumin is a naturally occurring compound which has been used in traditional medicine in India for a long time. This study investigated the ability of curcumin to inhibit the contractility of isolated caprine (goat) detrusor muscle. The ability of three concentrations of curcumin (30, 100 and 300 µ...

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Main Authors:	Manvizhi, S., Kumar, A., Shanthi, FX Margaret, Ernest, Kalpana
Format:	Online
Language:	English
Published:	Medknow Publications & Media Pvt Ltd 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442473/

id	pubmed-4442473
recordtype	oai_dc
spelling	pubmed-44424732015-05-25 Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle Manvizhi, S. Kumar, A. Shanthi, FX Margaret Ernest, Kalpana Short Communication Curcumin is a naturally occurring compound which has been used in traditional medicine in India for a long time. This study investigated the ability of curcumin to inhibit the contractility of isolated caprine (goat) detrusor muscle. The ability of three concentrations of curcumin (30, 100 and 300 µM) to inhibit the 100 µM acetylcholine-induced contractility of the isolated caprine urinary bladder detrusor muscle was investigated. The effect of raising the concentration of acetylcholine from 100, 200 and 400 µM to overcome the curcumin-induced inhibition of detrusor contractility and the effects of the reversal agents tetraethylammonium, a potassium channel blocker (100 µM), glibenclamide, an ATP-sensitive potassium channel blocker (10 µM), and propranolol, a beta adrenergic receptor blocker (1 µM), on the inhibitory effect of detrusor contractility was also studied. Curcumin caused a concentration-dependent inhibition of acetylcholine-induced contractility of the isolated detrusor muscle which was statistically significant at all three concentrations of curcumin used. This inhibition was partially overcome by raising the concentration of ACh to 200 and 400 µM. The inhibition was overcome by the concurrent administration of tetraethylammonium. Glibenclamide reversed the inhibitory effect of 100 µM curcumin, but not that of 300 µM curcumin. Propranolol reversed the inhibitory effect of 100 µM curcumin but not that of 300 µM curcumin. These results suggest that curcumin inhibited the contractions of the isolated detrusor muscle. The results further suggest that the inhibitory effect is mediated by various mechanisms: stimulation of beta adrenergic receptors; an anticholinergic effect; and the opening of ATP-sensitive potassium channels. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4442473/ /pubmed/26009657 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Manvizhi, S. Kumar, A. Shanthi, FX Margaret Ernest, Kalpana
spellingShingle	Manvizhi, S. Kumar, A. Shanthi, FX Margaret Ernest, Kalpana Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
author_facet	Manvizhi, S. Kumar, A. Shanthi, FX Margaret Ernest, Kalpana
author_sort	Manvizhi, S.
title	Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
title_short	Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
title_full	Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
title_fullStr	Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
title_full_unstemmed	Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle
title_sort	inhibitory effect of curcumin on the contractility of isolated caprine detrusor muscle
description	Curcumin is a naturally occurring compound which has been used in traditional medicine in India for a long time. This study investigated the ability of curcumin to inhibit the contractility of isolated caprine (goat) detrusor muscle. The ability of three concentrations of curcumin (30, 100 and 300 µM) to inhibit the 100 µM acetylcholine-induced contractility of the isolated caprine urinary bladder detrusor muscle was investigated. The effect of raising the concentration of acetylcholine from 100, 200 and 400 µM to overcome the curcumin-induced inhibition of detrusor contractility and the effects of the reversal agents tetraethylammonium, a potassium channel blocker (100 µM), glibenclamide, an ATP-sensitive potassium channel blocker (10 µM), and propranolol, a beta adrenergic receptor blocker (1 µM), on the inhibitory effect of detrusor contractility was also studied. Curcumin caused a concentration-dependent inhibition of acetylcholine-induced contractility of the isolated detrusor muscle which was statistically significant at all three concentrations of curcumin used. This inhibition was partially overcome by raising the concentration of ACh to 200 and 400 µM. The inhibition was overcome by the concurrent administration of tetraethylammonium. Glibenclamide reversed the inhibitory effect of 100 µM curcumin, but not that of 300 µM curcumin. Propranolol reversed the inhibitory effect of 100 µM curcumin but not that of 300 µM curcumin. These results suggest that curcumin inhibited the contractions of the isolated detrusor muscle. The results further suggest that the inhibitory effect is mediated by various mechanisms: stimulation of beta adrenergic receptors; an anticholinergic effect; and the opening of ATP-sensitive potassium channels.
publisher	Medknow Publications & Media Pvt Ltd
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442473/
_version_	1613227266752380928

Inhibitory Effect of Curcumin on the Contractility of Isolated Caprine Detrusor Muscle

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