Lentiviral Vectors Mediate Long-Term and High Efficiency Transgene Expression in HEK 293T cells

Objectives:Lentiviral vectors have been used successfully to rapidly produce decigram quantities of active recombinant proteins in mammalian cell lines. To optimize the protein production platform, the roles of Ubiquitous Chromatin Opening Element (UCOE), an insulator, and selected promoters were ev...

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Bibliographic Details
Main Authors: Mao, Yingying, Yan, Renhe, Li, Andrew, Zhang, Yanling, Li, Jinlong, Du, Hongyan, Chen, Baihong, Wei, Wenjin, Zhang, Yi, Sumners, Colin, Zheng, Haifa, Li, Hongwei
Format: Online
Language:English
Published: Ivyspring International Publisher 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4441065/