The Fecal Microbiome in Cats with Diarrhea

Recent studies have revealed that microbes play an important role in the pathogenesis of gastrointestinal (GI) diseases in various animal species, but only limited data is available about the microbiome in cats with GI disease. The aim of this study was to evaluate the fecal microbiome in cats with...

Full description

Bibliographic Details
Main Authors:	Suchodolski, Jan S., Foster, Mary L., Sohail, Muhammad U., Leutenegger, Christian, Queen, Erica V., Steiner, Jörg M., Marks, Stanley L.
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437779/

id	pubmed-4437779
recordtype	oai_dc
spelling	pubmed-44377792015-05-29 The Fecal Microbiome in Cats with Diarrhea Suchodolski, Jan S. Foster, Mary L. Sohail, Muhammad U. Leutenegger, Christian Queen, Erica V. Steiner, Jörg M. Marks, Stanley L. Research Article Recent studies have revealed that microbes play an important role in the pathogenesis of gastrointestinal (GI) diseases in various animal species, but only limited data is available about the microbiome in cats with GI disease. The aim of this study was to evaluate the fecal microbiome in cats with diarrhea. Fecal samples were obtained from healthy cats (n = 21) and cats with acute (n = 19) or chronic diarrhea (n = 29) and analyzed by sequencing of 16S rRNA genes, and PICRUSt was used to predict the functional gene content of the microbiome. Linear discriminant analysis (LDA) effect size (LEfSe) revealed significant differences in bacterial groups between healthy cats and cats with diarrhea. The order Burkholderiales, the families Enterobacteriaceae, and the genera Streptococcus and Collinsella were significantly increased in diarrheic cats. In contrast the order Campylobacterales, the family Bacteroidaceae, and the genera Megamonas, Helicobacter, and Roseburia were significantly increased in healthy cats. Phylum Bacteroidetes was significantly decreased in cats with chronic diarrhea (>21 days duration), while the class Erysipelotrichi and the genus Lactobacillus were significantly decreased in cats with acute diarrhea. The observed changes in bacterial groups were accompanied by significant differences in functional gene contents: metabolism of fatty acids, biosynthesis of glycosphingolipids, metabolism of biotin, metabolism of tryptophan, and ascorbate and aldarate metabolism, were all significantly (p<0.001) altered in cats with diarrhea. In conclusion, significant differences in the fecal microbiomes between healthy cats and cats with diarrhea were identified. This dysbiosis was accompanied by changes in bacterial functional gene categories. Future studies are warranted to evaluate if these microbial changes correlate with changes in fecal concentrations of microbial metabolites in cats with diarrhea for the identification of potential diagnostic or therapeutic targets. Public Library of Science 2015-05-19 /pmc/articles/PMC4437779/ /pubmed/25992741 http://dx.doi.org/10.1371/journal.pone.0127378 Text en © 2015 Suchodolski et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Suchodolski, Jan S. Foster, Mary L. Sohail, Muhammad U. Leutenegger, Christian Queen, Erica V. Steiner, Jörg M. Marks, Stanley L.
spellingShingle	Suchodolski, Jan S. Foster, Mary L. Sohail, Muhammad U. Leutenegger, Christian Queen, Erica V. Steiner, Jörg M. Marks, Stanley L. The Fecal Microbiome in Cats with Diarrhea
author_facet	Suchodolski, Jan S. Foster, Mary L. Sohail, Muhammad U. Leutenegger, Christian Queen, Erica V. Steiner, Jörg M. Marks, Stanley L.
author_sort	Suchodolski, Jan S.
title	The Fecal Microbiome in Cats with Diarrhea
title_short	The Fecal Microbiome in Cats with Diarrhea
title_full	The Fecal Microbiome in Cats with Diarrhea
title_fullStr	The Fecal Microbiome in Cats with Diarrhea
title_full_unstemmed	The Fecal Microbiome in Cats with Diarrhea
title_sort	fecal microbiome in cats with diarrhea
description	Recent studies have revealed that microbes play an important role in the pathogenesis of gastrointestinal (GI) diseases in various animal species, but only limited data is available about the microbiome in cats with GI disease. The aim of this study was to evaluate the fecal microbiome in cats with diarrhea. Fecal samples were obtained from healthy cats (n = 21) and cats with acute (n = 19) or chronic diarrhea (n = 29) and analyzed by sequencing of 16S rRNA genes, and PICRUSt was used to predict the functional gene content of the microbiome. Linear discriminant analysis (LDA) effect size (LEfSe) revealed significant differences in bacterial groups between healthy cats and cats with diarrhea. The order Burkholderiales, the families Enterobacteriaceae, and the genera Streptococcus and Collinsella were significantly increased in diarrheic cats. In contrast the order Campylobacterales, the family Bacteroidaceae, and the genera Megamonas, Helicobacter, and Roseburia were significantly increased in healthy cats. Phylum Bacteroidetes was significantly decreased in cats with chronic diarrhea (>21 days duration), while the class Erysipelotrichi and the genus Lactobacillus were significantly decreased in cats with acute diarrhea. The observed changes in bacterial groups were accompanied by significant differences in functional gene contents: metabolism of fatty acids, biosynthesis of glycosphingolipids, metabolism of biotin, metabolism of tryptophan, and ascorbate and aldarate metabolism, were all significantly (p<0.001) altered in cats with diarrhea. In conclusion, significant differences in the fecal microbiomes between healthy cats and cats with diarrhea were identified. This dysbiosis was accompanied by changes in bacterial functional gene categories. Future studies are warranted to evaluate if these microbial changes correlate with changes in fecal concentrations of microbial metabolites in cats with diarrhea for the identification of potential diagnostic or therapeutic targets.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437779/
_version_	1613225670429638656

The Fecal Microbiome in Cats with Diarrhea

Similar Items