Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)

Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)

The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein...

Full description

Bibliographic Details
Main Authors: Xinli, Xiao, Lei, Peng
Format: Online
Language:English
Published: Taylor & Francis 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434096/
id pubmed-4434096
recordtype oai_dc
spelling pubmed-44340962015-05-25 Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus) Xinli, Xiao Lei, Peng Articles; Agriculture and Environmental Biotechnology The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein shares high homology with the Rab18 of cucumber (99%), muskmelon (98%), Morus notabilis (90%), tomato (89%), wine grape (89%) and potato (88%). Phylogenetic analysis revealed that watermelon Rab18 gene has a closer genetic relationship with Rab18 gene of cucumber and muskmelon. Tissue expression profile analysis indicated that watermelon Rab18 gene was highly expressed in root, stem and leaf, moderately expressed in flower and weakly expressed in fruit. Taylor & Francis 2015-03-04 2015-02-03 /pmc/articles/PMC4434096/ /pubmed/26019638 http://dx.doi.org/10.1080/13102818.2015.1008198 Text en © 2015 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Xinli, Xiao
Lei, Peng
spellingShingle Xinli, Xiao
Lei, Peng
Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
author_facet Xinli, Xiao
Lei, Peng
author_sort Xinli, Xiao
title Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
title_short Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
title_full Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
title_fullStr Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
title_full_unstemmed Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
title_sort molecular cloning, sequence characterization and expression pattern of rab18 gene from watermelon (citrullus lanatus)
description The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein shares high homology with the Rab18 of cucumber (99%), muskmelon (98%), Morus notabilis (90%), tomato (89%), wine grape (89%) and potato (88%). Phylogenetic analysis revealed that watermelon Rab18 gene has a closer genetic relationship with Rab18 gene of cucumber and muskmelon. Tissue expression profile analysis indicated that watermelon Rab18 gene was highly expressed in root, stem and leaf, moderately expressed in flower and weakly expressed in fruit.
publisher Taylor & Francis
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434096/
_version_ 1613224359777796096

Similar Items