Establishment of efficient regeneration protocol for three rapeseed cultivars
Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present st...
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| Format: | Online |
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Taylor & Francis
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433892/ |
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pubmed-44338922015-05-25 Establishment of efficient regeneration protocol for three rapeseed cultivars Darçın, Emine Selcen Kolsarıcı, Özer Yıldız, Mustafa Articles; Agriculture & Environmental Biotechnology Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present study was to establish an efficient protocol for regeneration of rapeseed. It was conducted in three stages. The first stage was to determine the effect of different concentrations (10%, 20%, 30% and 40%) of sodium hypochlorite solutions on seedling growth parameters. In the second stage, the effects of different growth media (Murashige and Skoog, MS, and Gamborg) and plant growth regulators (6-benzylaminopurine, 1-naphthaleneacetic acid and thidiazuron) at different concentrations on the regeneration capacity of stem explants of three rapeseed cultivars were investigated. In the last stage, the shoots of ‘Spok’ were cultured for three weeks on MS medium containing 1.5, 3 and 4.5 mg L−1 of indol butyric acid for rooting. The best results in germination, seedling growth and root length were obtained by using 10% disinfectant concentration for 25 minutes. The highest values for shoot regeneration were obtained from the stem explant cultured on MS medium containing 3 mg L−1 BAP and 0.2 mg L−1 NAA. It was found that MS containing 1.5 mg L−1 IBA was the most efficient medium for root formation. Taylor & Francis 2014-01-02 2014-06-04 /pmc/articles/PMC4433892/ /pubmed/26019485 http://dx.doi.org/10.1080/13102818.2014.901668 Text en © 2014 The Author(s). Published by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Darçın, Emine Selcen Kolsarıcı, Özer Yıldız, Mustafa |
| spellingShingle |
Darçın, Emine Selcen Kolsarıcı, Özer Yıldız, Mustafa Establishment of efficient regeneration protocol for three rapeseed cultivars |
| author_facet |
Darçın, Emine Selcen Kolsarıcı, Özer Yıldız, Mustafa |
| author_sort |
Darçın, Emine Selcen |
| title |
Establishment of efficient regeneration protocol for three rapeseed cultivars |
| title_short |
Establishment of efficient regeneration protocol for three rapeseed cultivars |
| title_full |
Establishment of efficient regeneration protocol for three rapeseed cultivars |
| title_fullStr |
Establishment of efficient regeneration protocol for three rapeseed cultivars |
| title_full_unstemmed |
Establishment of efficient regeneration protocol for three rapeseed cultivars |
| title_sort |
establishment of efficient regeneration protocol for three rapeseed cultivars |
| description |
Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present study was to establish an efficient protocol for regeneration of rapeseed. It was conducted in three stages. The first stage was to determine the effect of different concentrations (10%, 20%, 30% and 40%) of sodium hypochlorite solutions on seedling growth parameters. In the second stage, the effects of different growth media (Murashige and Skoog, MS, and Gamborg) and plant growth regulators (6-benzylaminopurine, 1-naphthaleneacetic acid and thidiazuron) at different concentrations on the regeneration capacity of stem explants of three rapeseed cultivars were investigated. In the last stage, the shoots of ‘Spok’ were cultured for three weeks on MS medium containing 1.5, 3 and 4.5 mg L−1 of indol butyric acid for rooting. The best results in germination, seedling growth and root length were obtained by using 10% disinfectant concentration for 25 minutes. The highest values for shoot regeneration were obtained from the stem explant cultured on MS medium containing 3 mg L−1 BAP and 0.2 mg L−1 NAA. It was found that MS containing 1.5 mg L−1 IBA was the most efficient medium for root formation. |
| publisher |
Taylor & Francis |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433892/ |
| _version_ |
1613224250183778304 |