Cell-free expression of a functional pore-only sodium channel

•Cell-free expression of functional bacterial Na channel in mg quantities achieved.•The described method can be adopted for efficient site-directed isotope labelling.•This high throughput production enables bio-spectroscopic investigations.

Bibliographic Details
Main Authors: Kovácsová, Gabriela, Gustavsson, Emil, Wang, Jiajun, Kreir, Mohamed, Peuker, Sebastian, Westenhoff, Sebastian
Format: Online
Language:English
Published: Academic Press 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430601/