Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass

Perennial ryegrass (Lolium perenne L.) produces high levels of fructans as a mixture of oligosaccharides and polysaccharides with different degrees of polymerization (DP). The present study describes the analysis of the compositional changes in the full spectrum of fructans, fructan distribution bet...

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Main Authors:	Abeynayake, Shamila W., Etzerodt, Thomas P., Jonavičienė, Kristina, Byrne, Stephen, Asp, Torben, Boelt, Birte
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4428078/

id	pubmed-4428078
recordtype	oai_dc
spelling	pubmed-44280782015-05-29 Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass Abeynayake, Shamila W. Etzerodt, Thomas P. Jonavičienė, Kristina Byrne, Stephen Asp, Torben Boelt, Birte Plant Science Perennial ryegrass (Lolium perenne L.) produces high levels of fructans as a mixture of oligosaccharides and polysaccharides with different degrees of polymerization (DP). The present study describes the analysis of the compositional changes in the full spectrum of fructans, fructan distribution between above ground biomass (top) and the roots, and the transcription of candidate genes involved in fructan metabolism during cold acclimation in perennial ryegrass variety “Veyo” and ecotype “Falster” from distinct geographical origins. We observed changes in fructan composition and induction of low-DP fructans, especially DP = 4, in both the top and the roots of “Veyo” and “Falster” in response to low-temperature stress. The accumulation of DP > 50 fructans was only apparent in the top tissues where the Lp1-FFT expression is higher compared to the roots in both “Veyo” and “Falster.” Our results also show the accumulation and depolymerization of fructans with different DP, together with the induction of genes encoding fructosyltransferases and fructan exohydrolases in both “Veyo” and “Falster” during cold acclimation, supporting the hypothesis that fructan synthesis and depolymerization occurring simultaneously. The ecotype “Falster,” adapted to cold climates, increased total fructan content and produced more DP > 7 fructans in the roots than the variety “Veyo,” adapted to warmer climates. This indicates that high-DP fructan accumulation in roots may be an adaptive trait for plant recovery after abiotic stresses. Frontiers Media S.A. 2015-05-12 /pmc/articles/PMC4428078/ /pubmed/26029229 http://dx.doi.org/10.3389/fpls.2015.00329 Text en Copyright © 2015 Abeynayake, Etzerodt, Jonavičienė, Byrne, Asp and Boelt. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Abeynayake, Shamila W. Etzerodt, Thomas P. Jonavičienė, Kristina Byrne, Stephen Asp, Torben Boelt, Birte
spellingShingle	Abeynayake, Shamila W. Etzerodt, Thomas P. Jonavičienė, Kristina Byrne, Stephen Asp, Torben Boelt, Birte Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
author_facet	Abeynayake, Shamila W. Etzerodt, Thomas P. Jonavičienė, Kristina Byrne, Stephen Asp, Torben Boelt, Birte
author_sort	Abeynayake, Shamila W.
title	Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
title_short	Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
title_full	Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
title_fullStr	Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
title_full_unstemmed	Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
title_sort	fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass
description	Perennial ryegrass (Lolium perenne L.) produces high levels of fructans as a mixture of oligosaccharides and polysaccharides with different degrees of polymerization (DP). The present study describes the analysis of the compositional changes in the full spectrum of fructans, fructan distribution between above ground biomass (top) and the roots, and the transcription of candidate genes involved in fructan metabolism during cold acclimation in perennial ryegrass variety “Veyo” and ecotype “Falster” from distinct geographical origins. We observed changes in fructan composition and induction of low-DP fructans, especially DP = 4, in both the top and the roots of “Veyo” and “Falster” in response to low-temperature stress. The accumulation of DP > 50 fructans was only apparent in the top tissues where the Lp1-FFT expression is higher compared to the roots in both “Veyo” and “Falster.” Our results also show the accumulation and depolymerization of fructans with different DP, together with the induction of genes encoding fructosyltransferases and fructan exohydrolases in both “Veyo” and “Falster” during cold acclimation, supporting the hypothesis that fructan synthesis and depolymerization occurring simultaneously. The ecotype “Falster,” adapted to cold climates, increased total fructan content and produced more DP > 7 fructans in the roots than the variety “Veyo,” adapted to warmer climates. This indicates that high-DP fructan accumulation in roots may be an adaptive trait for plant recovery after abiotic stresses.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4428078/
_version_	1613222158651097088

Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass

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