Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture

Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells w...

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Main Authors:	Crabbé, Aurélie, Liu, Yulong, Sarker, Shameema F., Bonenfant, Nicholas R., Barrila, Jennifer, Borg, Zachary D., Lee, James J., Weiss, Daniel J., Nickerson, Cheryl A.
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427280/

id	pubmed-4427280
recordtype	oai_dc
spelling	pubmed-44272802015-05-21 Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture Crabbé, Aurélie Liu, Yulong Sarker, Shameema F. Bonenfant, Nicholas R. Barrila, Jennifer Borg, Zachary D. Lee, James J. Weiss, Daniel J. Nickerson, Cheryl A. Research Article Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells. Public Library of Science 2015-05-11 /pmc/articles/PMC4427280/ /pubmed/25962111 http://dx.doi.org/10.1371/journal.pone.0126846 Text en © 2015 Crabbé et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Crabbé, Aurélie Liu, Yulong Sarker, Shameema F. Bonenfant, Nicholas R. Barrila, Jennifer Borg, Zachary D. Lee, James J. Weiss, Daniel J. Nickerson, Cheryl A.
spellingShingle	Crabbé, Aurélie Liu, Yulong Sarker, Shameema F. Bonenfant, Nicholas R. Barrila, Jennifer Borg, Zachary D. Lee, James J. Weiss, Daniel J. Nickerson, Cheryl A. Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
author_facet	Crabbé, Aurélie Liu, Yulong Sarker, Shameema F. Bonenfant, Nicholas R. Barrila, Jennifer Borg, Zachary D. Lee, James J. Weiss, Daniel J. Nickerson, Cheryl A.
author_sort	Crabbé, Aurélie
title	Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
title_short	Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
title_full	Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
title_fullStr	Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
title_full_unstemmed	Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
title_sort	recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture
description	Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427280/
_version_	1613221832806105088

Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture

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