Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*

Macrophages operate at the forefront of innate immunity and their discrimination of foreign versus “self” particles is critical for a number of responses including efficient pathogen killing, antigen presentation, and cytokine induction. In order to efficiently destroy the particles and detect poten...

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Main Authors:	Dill, Brian D., Gierlinski, Marek, Härtlova, Anetta, Arandilla, Alba González, Guo, Manman, Clarke, Rosemary G., Trost, Matthias
Format:	Online
Language:	English
Published:	The American Society for Biochemistry and Molecular Biology 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424403/

id	pubmed-4424403
recordtype	oai_dc
spelling	pubmed-44244032015-05-18 Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors* Dill, Brian D. Gierlinski, Marek Härtlova, Anetta Arandilla, Alba González Guo, Manman Clarke, Rosemary G. Trost, Matthias Research Macrophages operate at the forefront of innate immunity and their discrimination of foreign versus “self” particles is critical for a number of responses including efficient pathogen killing, antigen presentation, and cytokine induction. In order to efficiently destroy the particles and detect potential threats, macrophages express an array of receptors to sense and phagocytose prey particles. In this study, we accurately quantified a proteomic time-course of isolated phagosomes from murine bone marrow-derived macrophages induced by particles conjugated to seven different ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers. We identified a clear functional differentiation over the three timepoints and detected subtle differences between certain ligand-phagosomes, indicating that triggering of receptors through a single ligand type has mild, but distinct, effects on phagosome proteome and function. Moreover, our data shows that uptake of phosphatidylserine-coated beads induces an active repression of NF-κB immune responses upon Toll-like receptor (TLR)-activation by recruitment of anti-inflammatory regulators to the phagosome. This data shows for the first time a systematic time-course analysis of bone marrow-derived macrophages phagosomes and how phagosome fate is regulated by the receptors triggered for phagocytosis. The American Society for Biochemistry and Molecular Biology 2015-05 /pmc/articles/PMC4424403/ /pubmed/25755298 http://dx.doi.org/10.1074/mcp.M114.044594 Text en © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/3.0) .
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Dill, Brian D. Gierlinski, Marek Härtlova, Anetta Arandilla, Alba González Guo, Manman Clarke, Rosemary G. Trost, Matthias
spellingShingle	Dill, Brian D. Gierlinski, Marek Härtlova, Anetta Arandilla, Alba González Guo, Manman Clarke, Rosemary G. Trost, Matthias Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
author_facet	Dill, Brian D. Gierlinski, Marek Härtlova, Anetta Arandilla, Alba González Guo, Manman Clarke, Rosemary G. Trost, Matthias
author_sort	Dill, Brian D.
title	Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
title_short	Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
title_full	Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
title_fullStr	Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
title_full_unstemmed	Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*
title_sort	quantitative proteome analysis of temporally resolved phagosomes following uptake via key phagocytic receptors*
description	Macrophages operate at the forefront of innate immunity and their discrimination of foreign versus “self” particles is critical for a number of responses including efficient pathogen killing, antigen presentation, and cytokine induction. In order to efficiently destroy the particles and detect potential threats, macrophages express an array of receptors to sense and phagocytose prey particles. In this study, we accurately quantified a proteomic time-course of isolated phagosomes from murine bone marrow-derived macrophages induced by particles conjugated to seven different ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers. We identified a clear functional differentiation over the three timepoints and detected subtle differences between certain ligand-phagosomes, indicating that triggering of receptors through a single ligand type has mild, but distinct, effects on phagosome proteome and function. Moreover, our data shows that uptake of phosphatidylserine-coated beads induces an active repression of NF-κB immune responses upon Toll-like receptor (TLR)-activation by recruitment of anti-inflammatory regulators to the phagosome. This data shows for the first time a systematic time-course analysis of bone marrow-derived macrophages phagosomes and how phagosome fate is regulated by the receptors triggered for phagocytosis.
publisher	The American Society for Biochemistry and Molecular Biology
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424403/
_version_	1613220890676297728

Quantitative Proteome Analysis of Temporally Resolved Phagosomes Following Uptake Via Key Phagocytic Receptors*

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