BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector
Nanotechnology has made a significant impact on the development of nanomedicine. Nonviral vectors have been attracting more attention for the advantage of biosafety in gene delivery. Polyethylenimine (PEI)-conjugated chitosan (chitosan-g-PEI) emerged as a promising nonviral vector and has been demon...
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| Format: | Online |
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Springer US
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420764/ |
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pubmed-44207642015-05-14 BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector Yue, Jianhui Wu, Jun Liu, Di Zhao, Xiaoli Lu, William W Nano Express Nanotechnology has made a significant impact on the development of nanomedicine. Nonviral vectors have been attracting more attention for the advantage of biosafety in gene delivery. Polyethylenimine (PEI)-conjugated chitosan (chitosan-g-PEI) emerged as a promising nonviral vector and has been demonstrated in many tumor cells. However, there is a lack of study focused on the behavior of this vector in stem cells which hold great potential in regenerative medicine. Therefore, in this study, in vitro gene delivering effect of chitosan-g-PEI was investigated in bone marrow stem cells. pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level. Chitosan-g-PEI-mediated gene transfer showed 17.2% of transfection efficiency and more than 80% of cell viability in stem cells. These values were higher than that of PEI. The expression of the delivered BMP2 gene in stem cells enhanced the osteogenic differentiation. These results demonstrated that chitosan-g-PEI is capable of applying in delivering gene to stem cells and providing potential applications in stem cell-based gene therapy. Springer US 2015-04-29 /pmc/articles/PMC4420764/ /pubmed/25977673 http://dx.doi.org/10.1186/s11671-015-0906-3 Text en © Yue et al.; licensee Springer. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Yue, Jianhui Wu, Jun Liu, Di Zhao, Xiaoli Lu, William W |
| spellingShingle |
Yue, Jianhui Wu, Jun Liu, Di Zhao, Xiaoli Lu, William W BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| author_facet |
Yue, Jianhui Wu, Jun Liu, Di Zhao, Xiaoli Lu, William W |
| author_sort |
Yue, Jianhui |
| title |
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| title_short |
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| title_full |
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| title_fullStr |
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| title_full_unstemmed |
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector |
| title_sort |
bmp2 gene delivery to bone mesenchymal stem cell by chitosan-g-pei nonviral vector |
| description |
Nanotechnology has made a significant impact on the development of nanomedicine. Nonviral vectors have been attracting more attention for the advantage of biosafety in gene delivery. Polyethylenimine (PEI)-conjugated chitosan (chitosan-g-PEI) emerged as a promising nonviral vector and has been demonstrated in many tumor cells. However, there is a lack of study focused on the behavior of this vector in stem cells which hold great potential in regenerative medicine. Therefore, in this study, in vitro gene delivering effect of chitosan-g-PEI was investigated in bone marrow stem cells. pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level. Chitosan-g-PEI-mediated gene transfer showed 17.2% of transfection efficiency and more than 80% of cell viability in stem cells. These values were higher than that of PEI. The expression of the delivered BMP2 gene in stem cells enhanced the osteogenic differentiation. These results demonstrated that chitosan-g-PEI is capable of applying in delivering gene to stem cells and providing potential applications in stem cell-based gene therapy. |
| publisher |
Springer US |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420764/ |
| _version_ |
1613219502896447488 |