A simplified purification protocol for recombinant adeno-associated virus vectors

We describe a new rapid, low cost, and scalable method for purification of various recombinant adeno-associated viruses (rAAVs) from the lysates of producer cells of either mammalian or insect origin. The method takes advantage of two general biochemical properties of all characterized AAV serotypes...

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Bibliographic Details
Main Authors: Potter, Mark, Lins, Bridget, Mietzsch, Mario, Heilbronn, Regine, Van Vliet, Kim, Chipman, Paul, Agbandje-McKenna, Mavis, Cleaver, Brian D, Clément, Nathalie, Byrne, Barry J, Zolotukhin, Sergei
Format: Online
Language:English
Published: Nature Publishing Group 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420252/