| Summary: | The aim of this research was to investigate the antiproliferative and
anticholinesterase activities of 11 extracts from 5 Annonaceae species in
vitro. Antiproliferative activity was assessed using 10 human cancer cell
lines. Thin-layer chromatography and a microplate assay were used to screen the
extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The
chemical compositions of the active extracts were investigated using high performance
liquid chromatography. Eleven extracts obtained from five Annonaceae plant species
were active and were particularly effective against the UA251, NCI-470 lung, HT-29,
NCI/ADR, and K-562 cell lines with growth inhibition (GI50) values of
0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL, respectively. In
addition, the Annona crassiflora and A. coriacea
seed extracts were the most active among the tested extracts and the most effective
against the tumor cell lines, with GI50 values below 8.90 µg/mL. The
A. cacans extract displayed the lowest activity. Based on the
microplate assay, the percent AchE inhibition of the extracts ranged from 12 to 52%,
and the A. coriacea seed extract resulted in the greatest inhibition
(52%). Caffeic acid, sinapic acid, and rutin were present at higher concentrations in
the A. crassiflora seed samples. The A. coriacea
seeds contained ferulic and sinapic acid. Overall, the results indicated that
A. crassiflora and A. coriacea extracts have
antiproliferative and anticholinesterase properties, which opens up new possibilities
for alternative pharmacotherapy drugs.
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