Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis

During meiosis homologous chromosomes undergo crossover recombination. Sequence differences between homologs can locally inhibit crossovers. Despite this, nucleotide diversity and population-scaled recombination are positively correlated in eukaryote genomes. To investigate interactions between hete...

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Main Authors:	Ziolkowski, Piotr A, Berchowitz, Luke E, Lambing, Christophe, Yelina, Nataliya E, Zhao, Xiaohui, Kelly, Krystyna A, Choi, Kyuha, Ziolkowska, Liliana, June, Viviana, Sanchez-Moran, Eugenio, Franklin, Chris, Copenhaver, Gregory P, Henderson, Ian R
Format:	Online
Language:	English
Published:	eLife Sciences Publications, Ltd 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407271/

id	pubmed-4407271
recordtype	oai_dc
spelling	pubmed-44072712015-04-24 Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis Ziolkowski, Piotr A Berchowitz, Luke E Lambing, Christophe Yelina, Nataliya E Zhao, Xiaohui Kelly, Krystyna A Choi, Kyuha Ziolkowska, Liliana June, Viviana Sanchez-Moran, Eugenio Franklin, Chris Copenhaver, Gregory P Henderson, Ian R Genes and Chromosomes During meiosis homologous chromosomes undergo crossover recombination. Sequence differences between homologs can locally inhibit crossovers. Despite this, nucleotide diversity and population-scaled recombination are positively correlated in eukaryote genomes. To investigate interactions between heterozygosity and recombination we crossed Arabidopsis lines carrying fluorescent crossover reporters to 32 diverse accessions and observed hybrids with significantly higher and lower crossovers than homozygotes. Using recombinant populations derived from these crosses we observed that heterozygous regions increase crossovers when juxtaposed with homozygous regions, which reciprocally decrease. Total crossovers measured by chiasmata were unchanged when heterozygosity was varied, consistent with homeostatic control. We tested the effects of heterozygosity in mutants where the balance of interfering and non-interfering crossover repair is altered. Crossover remodeling at homozygosity-heterozygosity junctions requires interference, and non-interfering repair is inefficient in heterozygous regions. As a consequence, heterozygous regions show stronger crossover interference. Our findings reveal how varying homolog polymorphism patterns can shape meiotic recombination. eLife Sciences Publications, Ltd 2015-03-27 /pmc/articles/PMC4407271/ /pubmed/25815584 http://dx.doi.org/10.7554/eLife.03708 Text en © 2015, Ziolkowski et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ziolkowski, Piotr A Berchowitz, Luke E Lambing, Christophe Yelina, Nataliya E Zhao, Xiaohui Kelly, Krystyna A Choi, Kyuha Ziolkowska, Liliana June, Viviana Sanchez-Moran, Eugenio Franklin, Chris Copenhaver, Gregory P Henderson, Ian R
spellingShingle	Ziolkowski, Piotr A Berchowitz, Luke E Lambing, Christophe Yelina, Nataliya E Zhao, Xiaohui Kelly, Krystyna A Choi, Kyuha Ziolkowska, Liliana June, Viviana Sanchez-Moran, Eugenio Franklin, Chris Copenhaver, Gregory P Henderson, Ian R Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
author_facet	Ziolkowski, Piotr A Berchowitz, Luke E Lambing, Christophe Yelina, Nataliya E Zhao, Xiaohui Kelly, Krystyna A Choi, Kyuha Ziolkowska, Liliana June, Viviana Sanchez-Moran, Eugenio Franklin, Chris Copenhaver, Gregory P Henderson, Ian R
author_sort	Ziolkowski, Piotr A
title	Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
title_short	Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
title_full	Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
title_fullStr	Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
title_full_unstemmed	Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis
title_sort	juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during arabidopsis meiosis
description	During meiosis homologous chromosomes undergo crossover recombination. Sequence differences between homologs can locally inhibit crossovers. Despite this, nucleotide diversity and population-scaled recombination are positively correlated in eukaryote genomes. To investigate interactions between heterozygosity and recombination we crossed Arabidopsis lines carrying fluorescent crossover reporters to 32 diverse accessions and observed hybrids with significantly higher and lower crossovers than homozygotes. Using recombinant populations derived from these crosses we observed that heterozygous regions increase crossovers when juxtaposed with homozygous regions, which reciprocally decrease. Total crossovers measured by chiasmata were unchanged when heterozygosity was varied, consistent with homeostatic control. We tested the effects of heterozygosity in mutants where the balance of interfering and non-interfering crossover repair is altered. Crossover remodeling at homozygosity-heterozygosity junctions requires interference, and non-interfering repair is inefficient in heterozygous regions. As a consequence, heterozygous regions show stronger crossover interference. Our findings reveal how varying homolog polymorphism patterns can shape meiotic recombination.
publisher	eLife Sciences Publications, Ltd
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407271/
_version_	1613214776368824320

Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis

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