Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers

Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maint...

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Main Authors:	Ji, Jiao-Jiao, Huang, Wei, Li, Zheng, Chai, Wei-Guo, Yin, Yan-Xu, Li, Da-Wei, Gong, Zhen-Hui
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406146/

id	pubmed-4406146
recordtype	oai_dc
spelling	pubmed-44061462015-05-07 Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers Ji, Jiao-Jiao Huang, Wei Li, Zheng Chai, Wei-Guo Yin, Yan-Xu Li, Da-Wei Gong, Zhen-Hui Plant Science Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific expression of the orf507 gene in the pepper maintainer line found that transformants were morphologically similar to untransformed and transformed control plants, but had shrunken anthers that showed little dehiscence and fewer pollen grains with lower germination rate and higher naturally damaged rate. These characters were different from those of CMS line which does not produce any pollen grains. Meanwhile a pollination test using transformants as the male parent set few fruit and there were few seeds in the limited number of fruits. At the tetrad stage, ablation of the tapetal cell induced by premature programmed cell death (PCD) occurred in the transformants and the microspores were distorted and degraded at the mononuclear stage. Stable transmission of induced semi-male sterility was confirmed by a test cross. In addition, expression of orf507 in the maintainer lines seemed to inhibit expression of atp6-2 to a certain extent, and lead to the increase of the activity of cytochrome c oxidase and the ATP hydrolysis of the mitochondrial F1Fo-ATP synthase. These results introduce the premature PCD caused by orf507 gene in tapetal cells and semi-male sterility, but not complete male sterility. Frontiers Media S.A. 2015-04-22 /pmc/articles/PMC4406146/ /pubmed/25954296 http://dx.doi.org/10.3389/fpls.2015.00272 Text en Copyright © 2015 Ji, Huang, Li, Chai, Yin, Li and Gong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ji, Jiao-Jiao Huang, Wei Li, Zheng Chai, Wei-Guo Yin, Yan-Xu Li, Da-Wei Gong, Zhen-Hui
spellingShingle	Ji, Jiao-Jiao Huang, Wei Li, Zheng Chai, Wei-Guo Yin, Yan-Xu Li, Da-Wei Gong, Zhen-Hui Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
author_facet	Ji, Jiao-Jiao Huang, Wei Li, Zheng Chai, Wei-Guo Yin, Yan-Xu Li, Da-Wei Gong, Zhen-Hui
author_sort	Ji, Jiao-Jiao
title	Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_short	Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_full	Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_fullStr	Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_full_unstemmed	Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_sort	tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
description	Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific expression of the orf507 gene in the pepper maintainer line found that transformants were morphologically similar to untransformed and transformed control plants, but had shrunken anthers that showed little dehiscence and fewer pollen grains with lower germination rate and higher naturally damaged rate. These characters were different from those of CMS line which does not produce any pollen grains. Meanwhile a pollination test using transformants as the male parent set few fruit and there were few seeds in the limited number of fruits. At the tetrad stage, ablation of the tapetal cell induced by premature programmed cell death (PCD) occurred in the transformants and the microspores were distorted and degraded at the mononuclear stage. Stable transmission of induced semi-male sterility was confirmed by a test cross. In addition, expression of orf507 in the maintainer lines seemed to inhibit expression of atp6-2 to a certain extent, and lead to the increase of the activity of cytochrome c oxidase and the ATP hydrolysis of the mitochondrial F1Fo-ATP synthase. These results introduce the premature PCD caused by orf507 gene in tapetal cells and semi-male sterility, but not complete male sterility.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406146/
_version_	1613214392016437248

Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers

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