RNA chaperones buffer deleterious mutations in E. coli

RNA chaperones buffer deleterious mutations in E. coli

Both proteins and RNAs can misfold into non-functional conformations. Protein chaperones promote native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutations that compromise protein structure and function. Here, we show that RNA chaperones can also act as mu...

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Main Authors: Rudan, Marina, Schneider, Dominique, Warnecke, Tobias, Krisko, Anita
Format: Online
Language:English
Published: eLife Sciences Publications, Ltd 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402597/
id pubmed-4402597
recordtype oai_dc
spelling pubmed-44025972015-04-22 RNA chaperones buffer deleterious mutations in E. coli Rudan, Marina Schneider, Dominique Warnecke, Tobias Krisko, Anita Genomics and Evolutionary Biology Both proteins and RNAs can misfold into non-functional conformations. Protein chaperones promote native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutations that compromise protein structure and function. Here, we show that RNA chaperones can also act as mutation buffers that enhance organismal fitness. Using competition assays, we demonstrate that overexpression of select RNA chaperones, including three DEAD box RNA helicases (DBRHs) (CsdA, SrmB, RhlB) and the cold shock protein CspA, improves fitness of two independently evolved Escherichia coli mutator strains that have accumulated deleterious mutations during short- and long-term laboratory evolution. We identify strain-specific mutations that are deleterious and subject to buffering when introduced individually into the ancestral genotype. For DBRHs, we show that buffering requires helicase activity, implicating RNA structural remodelling in the buffering process. Our results suggest that RNA chaperones might play a fundamental role in RNA evolution and evolvability. eLife Sciences Publications, Ltd 2015-03-25 /pmc/articles/PMC4402597/ /pubmed/25806682 http://dx.doi.org/10.7554/eLife.04745 Text en © 2015, Rudan et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Rudan, Marina
Schneider, Dominique
Warnecke, Tobias
Krisko, Anita
spellingShingle Rudan, Marina
Schneider, Dominique
Warnecke, Tobias
Krisko, Anita
RNA chaperones buffer deleterious mutations in E. coli
author_facet Rudan, Marina
Schneider, Dominique
Warnecke, Tobias
Krisko, Anita
author_sort Rudan, Marina
title RNA chaperones buffer deleterious mutations in E. coli
title_short RNA chaperones buffer deleterious mutations in E. coli
title_full RNA chaperones buffer deleterious mutations in E. coli
title_fullStr RNA chaperones buffer deleterious mutations in E. coli
title_full_unstemmed RNA chaperones buffer deleterious mutations in E. coli
title_sort rna chaperones buffer deleterious mutations in e. coli
description Both proteins and RNAs can misfold into non-functional conformations. Protein chaperones promote native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutations that compromise protein structure and function. Here, we show that RNA chaperones can also act as mutation buffers that enhance organismal fitness. Using competition assays, we demonstrate that overexpression of select RNA chaperones, including three DEAD box RNA helicases (DBRHs) (CsdA, SrmB, RhlB) and the cold shock protein CspA, improves fitness of two independently evolved Escherichia coli mutator strains that have accumulated deleterious mutations during short- and long-term laboratory evolution. We identify strain-specific mutations that are deleterious and subject to buffering when introduced individually into the ancestral genotype. For DBRHs, we show that buffering requires helicase activity, implicating RNA structural remodelling in the buffering process. Our results suggest that RNA chaperones might play a fundamental role in RNA evolution and evolvability.
publisher eLife Sciences Publications, Ltd
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402597/
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