Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for the treatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete s...

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Main Authors:	Zhang, Shu-quan, Wu, Min-fei, Piao, Zhe, Yao, Jin, Li, Ji-hai, Wang, Xin-gang, Liu, Jun
Format:	Online
Language:	English
Published:	Medknow Publications & Media Pvt Ltd 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392670/

id	pubmed-4392670
recordtype	oai_dc
spelling	pubmed-43926702015-04-16 Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats Zhang, Shu-quan Wu, Min-fei Piao, Zhe Yao, Jin Li, Ji-hai Wang, Xin-gang Liu, Jun Research Article Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for the treatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-labeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-labeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horseradish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined withSchwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function. Medknow Publications & Media Pvt Ltd 2015-02 /pmc/articles/PMC4392670/ /pubmed/25883621 http://dx.doi.org/10.4103/1673-5374.152376 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhang, Shu-quan Wu, Min-fei Piao, Zhe Yao, Jin Li, Ji-hai Wang, Xin-gang Liu, Jun
spellingShingle	Zhang, Shu-quan Wu, Min-fei Piao, Zhe Yao, Jin Li, Ji-hai Wang, Xin-gang Liu, Jun Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
author_facet	Zhang, Shu-quan Wu, Min-fei Piao, Zhe Yao, Jin Li, Ji-hai Wang, Xin-gang Liu, Jun
author_sort	Zhang, Shu-quan
title	Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
title_short	Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
title_full	Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
title_fullStr	Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
title_full_unstemmed	Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats
title_sort	edaravone combined with schwann cell transplantation may repair spinal cord injury in rats
description	Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for the treatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-labeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-labeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horseradish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined withSchwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function.
publisher	Medknow Publications & Media Pvt Ltd
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392670/
_version_	1613210138203652096

Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

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