The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study

As there is limited knowledge regarding the longitudinal development and early ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year, we sought to evaluate the changes in proportion of naïve (thymic and central) and regulatory (resting and activated) CD4+ T-cell popula...

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Main Authors:	Collier, Fiona M, Tang, Mimi L K, Martino, David, Saffery, Richard, Carlin, John, Jachno, Kim, Ranganathan, Sarath, Burgner, David, Allen, Katrina J, Vuillermin, Peter, Ponsonby, Anne-Louise
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386616/

id	pubmed-4386616
recordtype	oai_dc
spelling	pubmed-43866162015-04-09 The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study Collier, Fiona M Tang, Mimi L K Martino, David Saffery, Richard Carlin, John Jachno, Kim Ranganathan, Sarath Burgner, David Allen, Katrina J Vuillermin, Peter Ponsonby, Anne-Louise Original Article As there is limited knowledge regarding the longitudinal development and early ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year, we sought to evaluate the changes in proportion of naïve (thymic and central) and regulatory (resting and activated) CD4+ T-cell populations during the first postnatal year. Blood samples were collected and analyzed at birth, 6 and 12 months of age from a population-derived sample of 130 infants. The proportion of naïve and regulatory CD4+ T-cell populations was determined by flow cytometry, and the thymic and central naïve populations were sorted and their phenotype confirmed by relative expression of T cell-receptor excision circle DNA (TREC). At birth, the majority (94%) of CD4+ T cells were naïve (CD45RA+), and of these, ~80% had a thymic naïve phenotype (CD31+ and high TREC), with the remainder already central naïve cells (CD31− and low TREC). During the first year of life, the naïve CD4+ T cells retained an overall thymic phenotype but decreased steadily. From birth to 6 months of age, the proportion of both resting naïve T regulatory cells (rTreg; CD4+CD45RA+FoxP3+) and activated Treg (aTreg, CD4+CD45RA−FoxP3high) increased markedly. The ratio of thymic to central naïve CD4+ T cells was lower in males throughout the first postnatal year indicating early sexual dimorphism in immune development. This longitudinal study defines proportions of CD4+ T-cell populations during the first year of postnatal life that provide a better understanding of normal immune development. Nature Publishing Group 2015-03-27 /pmc/articles/PMC4386616/ /pubmed/25859389 http://dx.doi.org/10.1038/cti.2015.2 Text en Copyright © 2015 Australasian Society for Immunology Inc. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Collier, Fiona M Tang, Mimi L K Martino, David Saffery, Richard Carlin, John Jachno, Kim Ranganathan, Sarath Burgner, David Allen, Katrina J Vuillermin, Peter Ponsonby, Anne-Louise
spellingShingle	Collier, Fiona M Tang, Mimi L K Martino, David Saffery, Richard Carlin, John Jachno, Kim Ranganathan, Sarath Burgner, David Allen, Katrina J Vuillermin, Peter Ponsonby, Anne-Louise The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
author_facet	Collier, Fiona M Tang, Mimi L K Martino, David Saffery, Richard Carlin, John Jachno, Kim Ranganathan, Sarath Burgner, David Allen, Katrina J Vuillermin, Peter Ponsonby, Anne-Louise
author_sort	Collier, Fiona M
title	The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
title_short	The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
title_full	The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
title_fullStr	The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
title_full_unstemmed	The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study
title_sort	ontogeny of naïve and regulatory cd4+ t-cell subsets during the first postnatal year: a cohort study
description	As there is limited knowledge regarding the longitudinal development and early ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year, we sought to evaluate the changes in proportion of naïve (thymic and central) and regulatory (resting and activated) CD4+ T-cell populations during the first postnatal year. Blood samples were collected and analyzed at birth, 6 and 12 months of age from a population-derived sample of 130 infants. The proportion of naïve and regulatory CD4+ T-cell populations was determined by flow cytometry, and the thymic and central naïve populations were sorted and their phenotype confirmed by relative expression of T cell-receptor excision circle DNA (TREC). At birth, the majority (94%) of CD4+ T cells were naïve (CD45RA+), and of these, ~80% had a thymic naïve phenotype (CD31+ and high TREC), with the remainder already central naïve cells (CD31− and low TREC). During the first year of life, the naïve CD4+ T cells retained an overall thymic phenotype but decreased steadily. From birth to 6 months of age, the proportion of both resting naïve T regulatory cells (rTreg; CD4+CD45RA+FoxP3+) and activated Treg (aTreg, CD4+CD45RA−FoxP3high) increased markedly. The ratio of thymic to central naïve CD4+ T cells was lower in males throughout the first postnatal year indicating early sexual dimorphism in immune development. This longitudinal study defines proportions of CD4+ T-cell populations during the first year of postnatal life that provide a better understanding of normal immune development.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386616/
_version_	1613208047366176768

The ontogeny of naïve and regulatory CD4+ T-cell subsets during the first postnatal year: a cohort study

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