Encoding and decoding spatio-temporal information for super-resolution microscopy

The challenge of increasing the spatial resolution of an optical microscope beyond the diffraction limit can be reduced to a spectroscopy task by proper manipulation of the molecular states. The nanoscale spatial distribution of the molecules inside the detection volume of a scanning microscope can...

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Main Authors:	Lanzanò, Luca, Coto Hernández, Iván, Castello, Marco, Gratton, Enrico, Diaspro, Alberto, Vicidomini, Giuseppe
Format:	Online
Language:	English
Published:	Nature Pub. Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384168/

id	pubmed-4384168
recordtype	oai_dc
spelling	pubmed-43841682015-04-24 Encoding and decoding spatio-temporal information for super-resolution microscopy Lanzanò, Luca Coto Hernández, Iván Castello, Marco Gratton, Enrico Diaspro, Alberto Vicidomini, Giuseppe Article The challenge of increasing the spatial resolution of an optical microscope beyond the diffraction limit can be reduced to a spectroscopy task by proper manipulation of the molecular states. The nanoscale spatial distribution of the molecules inside the detection volume of a scanning microscope can be encoded within the fluorescence dynamics and decoded by resolving the signal into its dynamics components. Here we present a robust and general method to decode this information using phasor analysis. As an example of the application of this method, we optically generate spatially controlled gradients in the fluorescence lifetime by stimulated emission. Spatial resolution can be increased indefinitely by increasing the number of resolved dynamics components up to a maximum determined by the amount of noise. We demonstrate that the proposed method provides nanoscale imaging of subcellular structures, opening new routes in super-resolution microscopy based on the encoding/decoding of spatial information through manipulation of molecular dynamics. Nature Pub. Group 2015-04-02 /pmc/articles/PMC4384168/ /pubmed/25833391 http://dx.doi.org/10.1038/ncomms7701 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lanzanò, Luca Coto Hernández, Iván Castello, Marco Gratton, Enrico Diaspro, Alberto Vicidomini, Giuseppe
spellingShingle	Lanzanò, Luca Coto Hernández, Iván Castello, Marco Gratton, Enrico Diaspro, Alberto Vicidomini, Giuseppe Encoding and decoding spatio-temporal information for super-resolution microscopy
author_facet	Lanzanò, Luca Coto Hernández, Iván Castello, Marco Gratton, Enrico Diaspro, Alberto Vicidomini, Giuseppe
author_sort	Lanzanò, Luca
title	Encoding and decoding spatio-temporal information for super-resolution microscopy
title_short	Encoding and decoding spatio-temporal information for super-resolution microscopy
title_full	Encoding and decoding spatio-temporal information for super-resolution microscopy
title_fullStr	Encoding and decoding spatio-temporal information for super-resolution microscopy
title_full_unstemmed	Encoding and decoding spatio-temporal information for super-resolution microscopy
title_sort	encoding and decoding spatio-temporal information for super-resolution microscopy
description	The challenge of increasing the spatial resolution of an optical microscope beyond the diffraction limit can be reduced to a spectroscopy task by proper manipulation of the molecular states. The nanoscale spatial distribution of the molecules inside the detection volume of a scanning microscope can be encoded within the fluorescence dynamics and decoded by resolving the signal into its dynamics components. Here we present a robust and general method to decode this information using phasor analysis. As an example of the application of this method, we optically generate spatially controlled gradients in the fluorescence lifetime by stimulated emission. Spatial resolution can be increased indefinitely by increasing the number of resolved dynamics components up to a maximum determined by the amount of noise. We demonstrate that the proposed method provides nanoscale imaging of subcellular structures, opening new routes in super-resolution microscopy based on the encoding/decoding of spatial information through manipulation of molecular dynamics.
publisher	Nature Pub. Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384168/
_version_	1613207235452731392

Encoding and decoding spatio-temporal information for super-resolution microscopy

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