Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic

Detection of biomolecules in tissues provides contextual information and the possibility to assess the interaction of different cell types and markers. Routine qualitative assessment of immune- and oligonucleotide-based methods in research and the clinic has been associated with assay variability du...

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Main Authors:	Carvajal-Hausdorf, Daniel, Schalper, Kurt A., Neumeister, Veronique, Rimm, David L.
Format:	Online
Language:	English
Published:	2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383674/

id	pubmed-4383674
recordtype	oai_dc
spelling	pubmed-43836742015-10-01 Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic Carvajal-Hausdorf, Daniel Schalper, Kurt A. Neumeister, Veronique Rimm, David L. Article Detection of biomolecules in tissues provides contextual information and the possibility to assess the interaction of different cell types and markers. Routine qualitative assessment of immune- and oligonucleotide-based methods in research and the clinic has been associated with assay variability due to lack of stringent validation and subjective interpretation of results. As a result, the vast majority of in situ assays in clinical usage are non-quantitative and though useful often of questionable scientific validity. Here, we revisit the reporters and methods used for single- and multiplexed in situ visualization of protein and RNA. Then we examine methods for the use of quantitative platforms for in situ measurement of protein and mRNA levels. Finally, we discuss the challenges of the transition of these methods to the clinic and their potential role as tools for development of companion diagnostic tests. 2014-12-15 2015-04 /pmc/articles/PMC4383674/ /pubmed/25502176 http://dx.doi.org/10.1038/labinvest.2014.157 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Carvajal-Hausdorf, Daniel Schalper, Kurt A. Neumeister, Veronique Rimm, David L.
spellingShingle	Carvajal-Hausdorf, Daniel Schalper, Kurt A. Neumeister, Veronique Rimm, David L. Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
author_facet	Carvajal-Hausdorf, Daniel Schalper, Kurt A. Neumeister, Veronique Rimm, David L.
author_sort	Carvajal-Hausdorf, Daniel
title	Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
title_short	Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
title_full	Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
title_fullStr	Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
title_full_unstemmed	Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic
title_sort	quantitative measurement of cancer tissue biomarkers in the lab and in the clinic
description	Detection of biomolecules in tissues provides contextual information and the possibility to assess the interaction of different cell types and markers. Routine qualitative assessment of immune- and oligonucleotide-based methods in research and the clinic has been associated with assay variability due to lack of stringent validation and subjective interpretation of results. As a result, the vast majority of in situ assays in clinical usage are non-quantitative and though useful often of questionable scientific validity. Here, we revisit the reporters and methods used for single- and multiplexed in situ visualization of protein and RNA. Then we examine methods for the use of quantitative platforms for in situ measurement of protein and mRNA levels. Finally, we discuss the challenges of the transition of these methods to the clinic and their potential role as tools for development of companion diagnostic tests.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383674/
_version_	1613207028736458752

Quantitative Measurement of Cancer Tissue Biomarkers in the Lab and in the Clinic

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