Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice

In order to investigate the mechanisms by which puerarin from kudzu root extract regulates lipid metabolism, fifty mice were randomly assigned to five groups: normal diet, high-fat diet (HFD), and HFD containing 0.2%, 0.4% or 0.8% puerarin for 12 weeks. Body weight, intraperitioneal adipose tissue (...

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Main Authors:	Zheng, Guodong, Lin, Lezhen, Zhong, Shusheng, Zhang, Qingfeng, Li, Dongming
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378957/

id	pubmed-4378957
recordtype	oai_dc
spelling	pubmed-43789572015-04-09 Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice Zheng, Guodong Lin, Lezhen Zhong, Shusheng Zhang, Qingfeng Li, Dongming Research Article In order to investigate the mechanisms by which puerarin from kudzu root extract regulates lipid metabolism, fifty mice were randomly assigned to five groups: normal diet, high-fat diet (HFD), and HFD containing 0.2%, 0.4% or 0.8% puerarin for 12 weeks. Body weight, intraperitioneal adipose tissue (IPAT) weight, serum biochemical parameters, and hepatic and feces lipids were measured. Activity and mRNA and protein expressions of hepatic lipid metabolism-related enzymes were analyzed. Compared with HFD, 0.4% and 0.8% puerarin significantly decreased body and IPAT weight. There was a significant decrease in the serum and hepatic concentrations of total cholesterol, triglycerides and leptin in mice fed the 0.4% and 0.8% puerarin diets compared with HFD. Fatty acid synthase activity was suppressed in mice fed the 0.4% and 0.8% puerarin diets, while the activities of AMP-activated protein kinase (AMPK), carnitine acyltransferase (CAT) and hormone-sensitive lipase (HSL) were increased. mRNA expression of peroxisome proliferator-activated receptor γ 2 (PPARγ 2) was down-regulated in liver of mice fed the 0.8% diet compared with HFD, while mRNA expression of CAT and HSL was considerably up-regulated by 0.4% and 0.8% puerarin diets. The protein expression of PPARγ2 in liver was decreased and those of p-AMPK, HSL and p-HSL were increased in mice fed 0.4% and 0.8% puerarin diets. These results suggest that > 0.4% puerarin influenced the activity, mRNA and protein levels of hepatic lipid metabolism-related enzymes, decreasing serum and liver lipids, body weight gain and fat accumulation. Puerarin might be beneficial to prevent lifestyle-related diseases. Public Library of Science 2015-03-30 /pmc/articles/PMC4378957/ /pubmed/25822741 http://dx.doi.org/10.1371/journal.pone.0122925 Text en © 2015 Zheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zheng, Guodong Lin, Lezhen Zhong, Shusheng Zhang, Qingfeng Li, Dongming
spellingShingle	Zheng, Guodong Lin, Lezhen Zhong, Shusheng Zhang, Qingfeng Li, Dongming Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
author_facet	Zheng, Guodong Lin, Lezhen Zhong, Shusheng Zhang, Qingfeng Li, Dongming
author_sort	Zheng, Guodong
title	Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
title_short	Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
title_full	Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
title_fullStr	Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
title_full_unstemmed	Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice
title_sort	effects of puerarin on lipid accumulation and metabolism in high-fat diet-fed mice
description	In order to investigate the mechanisms by which puerarin from kudzu root extract regulates lipid metabolism, fifty mice were randomly assigned to five groups: normal diet, high-fat diet (HFD), and HFD containing 0.2%, 0.4% or 0.8% puerarin for 12 weeks. Body weight, intraperitioneal adipose tissue (IPAT) weight, serum biochemical parameters, and hepatic and feces lipids were measured. Activity and mRNA and protein expressions of hepatic lipid metabolism-related enzymes were analyzed. Compared with HFD, 0.4% and 0.8% puerarin significantly decreased body and IPAT weight. There was a significant decrease in the serum and hepatic concentrations of total cholesterol, triglycerides and leptin in mice fed the 0.4% and 0.8% puerarin diets compared with HFD. Fatty acid synthase activity was suppressed in mice fed the 0.4% and 0.8% puerarin diets, while the activities of AMP-activated protein kinase (AMPK), carnitine acyltransferase (CAT) and hormone-sensitive lipase (HSL) were increased. mRNA expression of peroxisome proliferator-activated receptor γ 2 (PPARγ 2) was down-regulated in liver of mice fed the 0.8% diet compared with HFD, while mRNA expression of CAT and HSL was considerably up-regulated by 0.4% and 0.8% puerarin diets. The protein expression of PPARγ2 in liver was decreased and those of p-AMPK, HSL and p-HSL were increased in mice fed 0.4% and 0.8% puerarin diets. These results suggest that > 0.4% puerarin influenced the activity, mRNA and protein levels of hepatic lipid metabolism-related enzymes, decreasing serum and liver lipids, body weight gain and fat accumulation. Puerarin might be beneficial to prevent lifestyle-related diseases.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378957/
_version_	1613205359946629120

Effects of Puerarin on Lipid Accumulation and Metabolism in High-Fat Diet-Fed Mice

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