High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas

Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new E...

Full description

Bibliographic Details
Main Authors:	Shen, Zhi-chao, Luo, Bing, Chen, Jian-ning, Chao, Yan, Shao, Chun-kui, Liu, Qian-qian, Wang, Yun
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4373760/

id	pubmed-4373760
recordtype	oai_dc
spelling	pubmed-43737602015-03-27 High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas Shen, Zhi-chao Luo, Bing Chen, Jian-ning Chao, Yan Shao, Chun-kui Liu, Qian-qian Wang, Yun Research Article Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new EBER variants (EB-8m and EB-10m) which were less common but relatively more frequent in NPC cases than healthy donors. In the present study, we determined the EBER variants in NPC cases and healthy donors from endemic and non-endemic areas of NPC within China and compared the EBER variants, in relation to the genotypes at BamHI F region (prototype F and f variant), between population groups and between two areas. According to the phylogenetic tree, four EBER variants (EB-6m, EB-8m, EB-10m and B95-8) were identified. EB-6m was dominant in all population groups except for endemic NPC group, in which EB-8m was dominant. EB-8m was more common in endemic NPC cases (82.0%, 41/50) than non-endemic NPC cases (33.7%, 32/95) (p<0.0001), and it was also more frequent in healthy donors from endemic area (32.4%, 24/74) than healthy donors from non-endemic area (1.1%, 1/92) (p<0.0001). More importantly, the EB-8m was more prevalent in NPC cases than healthy donors in both areas (p<0.0001). The f variant, which has been suggested to associate with endemic NPC, demonstrated preferential linkage with EB-8m in endemic isolates, however, the EB-8m variant seemed to be more specific to NPC isolates than f variant. These results reveal high prevalence of EBER EB-8m variant in endemic NPC cases, suggesting an association between NPC development and EBV isolates carrying EB-8m variant. Our finding identified a small healthy population group that shares the same viral strain which predominates in NPC cases. It could be interesting to carry extensive cohort studies following these individuals to evaluate the risk to develop NPC. Public Library of Science 2015-03-25 /pmc/articles/PMC4373760/ /pubmed/25807550 http://dx.doi.org/10.1371/journal.pone.0121420 Text en © 2015 Shen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Shen, Zhi-chao Luo, Bing Chen, Jian-ning Chao, Yan Shao, Chun-kui Liu, Qian-qian Wang, Yun
spellingShingle	Shen, Zhi-chao Luo, Bing Chen, Jian-ning Chao, Yan Shao, Chun-kui Liu, Qian-qian Wang, Yun High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
author_facet	Shen, Zhi-chao Luo, Bing Chen, Jian-ning Chao, Yan Shao, Chun-kui Liu, Qian-qian Wang, Yun
author_sort	Shen, Zhi-chao
title	High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
title_short	High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
title_full	High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
title_fullStr	High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
title_full_unstemmed	High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas
title_sort	high prevalence of the eber variant eb-8m in endemic nasopharyngeal carcinomas
description	Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new EBER variants (EB-8m and EB-10m) which were less common but relatively more frequent in NPC cases than healthy donors. In the present study, we determined the EBER variants in NPC cases and healthy donors from endemic and non-endemic areas of NPC within China and compared the EBER variants, in relation to the genotypes at BamHI F region (prototype F and f variant), between population groups and between two areas. According to the phylogenetic tree, four EBER variants (EB-6m, EB-8m, EB-10m and B95-8) were identified. EB-6m was dominant in all population groups except for endemic NPC group, in which EB-8m was dominant. EB-8m was more common in endemic NPC cases (82.0%, 41/50) than non-endemic NPC cases (33.7%, 32/95) (p<0.0001), and it was also more frequent in healthy donors from endemic area (32.4%, 24/74) than healthy donors from non-endemic area (1.1%, 1/92) (p<0.0001). More importantly, the EB-8m was more prevalent in NPC cases than healthy donors in both areas (p<0.0001). The f variant, which has been suggested to associate with endemic NPC, demonstrated preferential linkage with EB-8m in endemic isolates, however, the EB-8m variant seemed to be more specific to NPC isolates than f variant. These results reveal high prevalence of EBER EB-8m variant in endemic NPC cases, suggesting an association between NPC development and EBV isolates carrying EB-8m variant. Our finding identified a small healthy population group that shares the same viral strain which predominates in NPC cases. It could be interesting to carry extensive cohort studies following these individuals to evaluate the risk to develop NPC.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4373760/
_version_	1613203515190018048

High Prevalence of the EBER Variant EB-8m in Endemic Nasopharyngeal Carcinomas

Similar Items