Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However,...

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Main Authors: Balvan, Jan, Krizova, Aneta, Gumulec, Jaromir, Raudenska, Martina, Sladek, Zbysek, Sedlackova, Miroslava, Babula, Petr, Sztalmachova, Marketa, Kizek, Rene, Chmelik, Radim, Masarik, Michal
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372376/
id pubmed-4372376
recordtype oai_dc
spelling pubmed-43723762015-04-04 Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis Balvan, Jan Krizova, Aneta Gumulec, Jaromir Raudenska, Martina Sladek, Zbysek Sedlackova, Miroslava Babula, Petr Sztalmachova, Marketa Kizek, Rene Chmelik, Radim Masarik, Michal Research Article Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - “Multimodal holographic microscopy (MHM)”. The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI− phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis. Public Library of Science 2015-03-24 /pmc/articles/PMC4372376/ /pubmed/25803711 http://dx.doi.org/10.1371/journal.pone.0121674 Text en © 2015 Balvan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Balvan, Jan
Krizova, Aneta
Gumulec, Jaromir
Raudenska, Martina
Sladek, Zbysek
Sedlackova, Miroslava
Babula, Petr
Sztalmachova, Marketa
Kizek, Rene
Chmelik, Radim
Masarik, Michal
spellingShingle Balvan, Jan
Krizova, Aneta
Gumulec, Jaromir
Raudenska, Martina
Sladek, Zbysek
Sedlackova, Miroslava
Babula, Petr
Sztalmachova, Marketa
Kizek, Rene
Chmelik, Radim
Masarik, Michal
Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
author_facet Balvan, Jan
Krizova, Aneta
Gumulec, Jaromir
Raudenska, Martina
Sladek, Zbysek
Sedlackova, Miroslava
Babula, Petr
Sztalmachova, Marketa
Kizek, Rene
Chmelik, Radim
Masarik, Michal
author_sort Balvan, Jan
title Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
title_short Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
title_full Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
title_fullStr Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
title_full_unstemmed Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
title_sort multimodal holographic microscopy: distinction between apoptosis and oncosis
description Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - “Multimodal holographic microscopy (MHM)”. The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI− phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
publisher Public Library of Science
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372376/
_version_ 1613202998840786944

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