An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity

An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity

We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and...

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Main Authors: Fayon, Michael, Andrieux, Annick, Bara, Imane, Rebola, Muriel, Labbé, André, Marthan, Roger, Berger, Patrick
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370680/
id pubmed-4370680
recordtype oai_dc
spelling pubmed-43706802015-04-04 An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity Fayon, Michael Andrieux, Annick Bara, Imane Rebola, Muriel Labbé, André Marthan, Roger Berger, Patrick Research Article We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis. Public Library of Science 2015-03-23 /pmc/articles/PMC4370680/ /pubmed/25798916 http://dx.doi.org/10.1371/journal.pone.0122446 Text en © 2015 Fayon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Fayon, Michael
Andrieux, Annick
Bara, Imane
Rebola, Muriel
Labbé, André
Marthan, Roger
Berger, Patrick
spellingShingle Fayon, Michael
Andrieux, Annick
Bara, Imane
Rebola, Muriel
Labbé, André
Marthan, Roger
Berger, Patrick
An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
author_facet Fayon, Michael
Andrieux, Annick
Bara, Imane
Rebola, Muriel
Labbé, André
Marthan, Roger
Berger, Patrick
author_sort Fayon, Michael
title An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
title_short An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
title_full An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
title_fullStr An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
title_full_unstemmed An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity
title_sort age-wise comparison of human airway smooth muscle proliferative capacity
description We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis.
publisher Public Library of Science
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370680/
_version_ 1613202442737942528

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