Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models
Hirschsprung disease (HSCR) is a well-known congenital digestive disease that originates due to the developmental disorder of neural crest cells. MiR-206 is kown to have a relationship with digestive malfunctions. Therefore, we investigated whether or not miR-206 was involved in the pathogenesis of...
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Nature Publishing Group
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366810/ |
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pubmed-43668102015-03-31 Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models Sharan, Ankur Zhu, Hairong Xie, Hua Li, Hongxing Tang, Junwei Tang, Weibing Zhang, Hongwei Xia, Yankai Article Hirschsprung disease (HSCR) is a well-known congenital digestive disease that originates due to the developmental disorder of neural crest cells. MiR-206 is kown to have a relationship with digestive malfunctions. Therefore, we investigated whether or not miR-206 was involved in the pathogenesis of HSCR. qRT-PCR and Western blot assays were used to detect the expression levels of miRNA and mRNAs, and proteins in case and control tissue samples and two cell lines (293T and SH-SY5Y). The functions of miR-206 in vitro were measured by transwell assay, CCK8 assay and flow cytometry. Finally, we conducted dual-luciferase reporter assay to verify the connections between miR-206 and the target mRNA SDPR. Down-regulation of miR-206 was found in HSCR case tissue samples compared with controls, which was validated to be connected with the increased level of mRNA and protein of SDPR by qRT-PCR and dual-luciferase reporter assay. Moreover, miR-206 suppressed the cell migration and proliferation and silencing of SDPR could rescue the extent of the suppressing effects by miR-206 inhibitor. The findings suggest that miR-206 may play a significant role in the pathogenesis of HSCR, as well as inhibiting the cell migration and proliferation by targeting SDPR in disease models. Nature Publishing Group 2015-03-20 /pmc/articles/PMC4366810/ /pubmed/25792468 http://dx.doi.org/10.1038/srep09302 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Sharan, Ankur Zhu, Hairong Xie, Hua Li, Hongxing Tang, Junwei Tang, Weibing Zhang, Hongwei Xia, Yankai |
| spellingShingle |
Sharan, Ankur Zhu, Hairong Xie, Hua Li, Hongxing Tang, Junwei Tang, Weibing Zhang, Hongwei Xia, Yankai Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| author_facet |
Sharan, Ankur Zhu, Hairong Xie, Hua Li, Hongxing Tang, Junwei Tang, Weibing Zhang, Hongwei Xia, Yankai |
| author_sort |
Sharan, Ankur |
| title |
Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| title_short |
Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| title_full |
Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| title_fullStr |
Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| title_full_unstemmed |
Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models |
| title_sort |
down-regulation of mir-206 is associated with hirschsprung disease and suppresses cell migration and proliferation in cell models |
| description |
Hirschsprung disease (HSCR) is a well-known congenital digestive disease that originates due to the developmental disorder of neural crest cells. MiR-206 is kown to have a relationship with digestive malfunctions. Therefore, we investigated whether or not miR-206 was involved in the pathogenesis of HSCR. qRT-PCR and Western blot assays were used to detect the expression levels of miRNA and mRNAs, and proteins in case and control tissue samples and two cell lines (293T and SH-SY5Y). The functions of miR-206 in vitro were measured by transwell assay, CCK8 assay and flow cytometry. Finally, we conducted dual-luciferase reporter assay to verify the connections between miR-206 and the target mRNA SDPR. Down-regulation of miR-206 was found in HSCR case tissue samples compared with controls, which was validated to be connected with the increased level of mRNA and protein of SDPR by qRT-PCR and dual-luciferase reporter assay. Moreover, miR-206 suppressed the cell migration and proliferation and silencing of SDPR could rescue the extent of the suppressing effects by miR-206 inhibitor. The findings suggest that miR-206 may play a significant role in the pathogenesis of HSCR, as well as inhibiting the cell migration and proliferation by targeting SDPR in disease models. |
| publisher |
Nature Publishing Group |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366810/ |
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1613201177922502656 |