Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components

Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isola...

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Main Authors:	Li, Zhao-Qun, Zhang, Shuai, Luo, Jun-Yu, Wang, Chun-Yi, Lv, Li-Min, Dong, Shuang-Lin, Cui, Jin-Jie
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366804/

id	pubmed-4366804
recordtype	oai_dc
spelling	pubmed-43668042015-03-31 Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components Li, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Wang, Chun-Yi Lv, Li-Min Dong, Shuang-Lin Cui, Jin-Jie Article Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isolation. To investigate how the ratios of the pheromone components are differently regulated in the two species, we sequenced cDNA libraries from the pheromone glands of H. armigera and H. assulta. After assembly and annotation, we identified 108 and 93 transcripts putatively involved in pheromone biosynthesis, transport, and degradation in H. armigera and H. assulta, respectively. Semi-quantitative RT-PCR, qRT-PCR, phylogenetic, and mRNA abundance analyses suggested that some of these transcripts involved in the sex pheromone biosynthesis pathways perform. Based on these results, we postulate that the regulation of desaturases, KPSE and LPAQ, might be key factor regulating the opposite component ratios in the two sibling moths. In addition, our study has yielded large-scale sequence information for further studies and can be used to identify potential targets for the bio-control of these species by disrupting their sexual communication. Nature Publishing Group 2015-03-20 /pmc/articles/PMC4366804/ /pubmed/25792497 http://dx.doi.org/10.1038/srep09324 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Li, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Wang, Chun-Yi Lv, Li-Min Dong, Shuang-Lin Cui, Jin-Jie
spellingShingle	Li, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Wang, Chun-Yi Lv, Li-Min Dong, Shuang-Lin Cui, Jin-Jie Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
author_facet	Li, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Wang, Chun-Yi Lv, Li-Min Dong, Shuang-Lin Cui, Jin-Jie
author_sort	Li, Zhao-Qun
title	Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
title_short	Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
title_full	Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
title_fullStr	Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
title_full_unstemmed	Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components
title_sort	transcriptome comparison of the sex pheromone glands from two sibling helicoverpa species with opposite sex pheromone components
description	Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isolation. To investigate how the ratios of the pheromone components are differently regulated in the two species, we sequenced cDNA libraries from the pheromone glands of H. armigera and H. assulta. After assembly and annotation, we identified 108 and 93 transcripts putatively involved in pheromone biosynthesis, transport, and degradation in H. armigera and H. assulta, respectively. Semi-quantitative RT-PCR, qRT-PCR, phylogenetic, and mRNA abundance analyses suggested that some of these transcripts involved in the sex pheromone biosynthesis pathways perform. Based on these results, we postulate that the regulation of desaturases, KPSE and LPAQ, might be key factor regulating the opposite component ratios in the two sibling moths. In addition, our study has yielded large-scale sequence information for further studies and can be used to identify potential targets for the bio-control of these species by disrupting their sexual communication.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366804/
_version_	1613201174466396160

Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components

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