Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice

Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance t...

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Main Authors:	YAN, Yue, NIU, Lili, DENG, Jiabo, WANG, Qiang, YU, Jianqiu, ZHANG, Yizheng, WANG, Jianxi, CHEN, Jiao, WEI, Changhe, TAN, Xuemei
Format:	Online
Language:	English
Published:	The Japanese Society of Veterinary Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363021/

id	pubmed-4363021
recordtype	oai_dc
spelling	pubmed-43630212015-03-19 Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice YAN, Yue NIU, Lili DENG, Jiabo WANG, Qiang YU, Jianqiu ZHANG, Yizheng WANG, Jianxi CHEN, Jiao WEI, Changhe TAN, Xuemei Laboratory Animal Science Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance the development of antigen-specific immunity and vaccine efficacy. In this study, a giant panda IL-18 gene eukaryotic expression plasmid (pcAmIL-18) was constructed. Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively. They were subsequently injected with an attenuated CDV vaccine for dogs, and the induced humoral and cellular responses were evaluated. The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4+ and CD8+ cells. These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity. The Japanese Society of Veterinary Science 2014-11-14 2015-02 /pmc/articles/PMC4363021/ /pubmed/25399820 http://dx.doi.org/10.1292/jvms.14-0226 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	YAN, Yue NIU, Lili DENG, Jiabo WANG, Qiang YU, Jianqiu ZHANG, Yizheng WANG, Jianxi CHEN, Jiao WEI, Changhe TAN, Xuemei
spellingShingle	YAN, Yue NIU, Lili DENG, Jiabo WANG, Qiang YU, Jianqiu ZHANG, Yizheng WANG, Jianxi CHEN, Jiao WEI, Changhe TAN, Xuemei Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
author_facet	YAN, Yue NIU, Lili DENG, Jiabo WANG, Qiang YU, Jianqiu ZHANG, Yizheng WANG, Jianxi CHEN, Jiao WEI, Changhe TAN, Xuemei
author_sort	YAN, Yue
title	Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
title_short	Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
title_full	Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
title_fullStr	Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
title_full_unstemmed	Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice
title_sort	adjuvant effects of recombinant giant panda (ailuropoda melanoleuca) il-18 on the canine distemper disease vaccine in mice
description	Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance the development of antigen-specific immunity and vaccine efficacy. In this study, a giant panda IL-18 gene eukaryotic expression plasmid (pcAmIL-18) was constructed. Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively. They were subsequently injected with an attenuated CDV vaccine for dogs, and the induced humoral and cellular responses were evaluated. The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4+ and CD8+ cells. These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.
publisher	The Japanese Society of Veterinary Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363021/
_version_	1613199760514088960

Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice

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