SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

A prerequisite for reliable detection of low-density Plasmodium infections in malaria pre-elimination settings is the availability of ultra-sensitive and high-throughput molecular tools. We developed a SYBR Green real-time PCR restriction fragment length polymorphism assay (cytb-qPCR) targeting the...

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Bibliographic Details
Main Authors: Xu, Weiping, Morris, Ulrika, Aydin-Schmidt, Berit, Msellem, Mwinyi I., Shakely, Delér, Petzold, Max, Björkman, Anders, Mårtensson, Andreas
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361616/