Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis
Patients with ulcerative colitis (UC) have a high risk of developing colorectal cancer. The aim of the present study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the colonic mucosa of patients with UC. Colonic mucosal biopsy specimens were collected during colonoscopy from...
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D.A. Spandidos
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353785/ |
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pubmed-4353785 |
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pubmed-43537852015-03-16 Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis FAN, YUJING LIU, BINGRONG Articles Patients with ulcerative colitis (UC) have a high risk of developing colorectal cancer. The aim of the present study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the colonic mucosa of patients with UC. Colonic mucosal biopsy specimens were collected during colonoscopy from 30 patients with UC and 30 patients with normal findings as controls. The protein and mRNA expression levels of TLRs 1-4 and TLR9 were measured by immunohistochemistry and reverse transcription-quantitative polymerase chain reaction analysis, respectively. The results showed that the mRNA and protein expression of TLR2, TLR4 and TLR9, but not TLR1 and TLR3, was significantly increased in the colonic mucosa of patients with UC compared with that in the normal controls. TLR (TLR2, TLR4 and TLR9) immunoreactivity was found in the cytoplasm of epithelial cells in the mucosa, and occasionally in the endothelium of small vessels of the stromal tissues. In conclusion, TLR2, TLR4 and TLR9 expression may be important in the biological pathogenesis of UC. TLR alterations in the innate response system may contribute to the pathogenesis of UC. D.A. Spandidos 2015-04 2015-02-04 /pmc/articles/PMC4353785/ /pubmed/25780451 http://dx.doi.org/10.3892/etm.2015.2258 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
FAN, YUJING LIU, BINGRONG |
| spellingShingle |
FAN, YUJING LIU, BINGRONG Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| author_facet |
FAN, YUJING LIU, BINGRONG |
| author_sort |
FAN, YUJING |
| title |
Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| title_short |
Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| title_full |
Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| title_fullStr |
Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| title_full_unstemmed |
Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis |
| title_sort |
expression of toll-like receptors in the mucosa of patients with ulcerative colitis |
| description |
Patients with ulcerative colitis (UC) have a high risk of developing colorectal cancer. The aim of the present study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the colonic mucosa of patients with UC. Colonic mucosal biopsy specimens were collected during colonoscopy from 30 patients with UC and 30 patients with normal findings as controls. The protein and mRNA expression levels of TLRs 1-4 and TLR9 were measured by immunohistochemistry and reverse transcription-quantitative polymerase chain reaction analysis, respectively. The results showed that the mRNA and protein expression of TLR2, TLR4 and TLR9, but not TLR1 and TLR3, was significantly increased in the colonic mucosa of patients with UC compared with that in the normal controls. TLR (TLR2, TLR4 and TLR9) immunoreactivity was found in the cytoplasm of epithelial cells in the mucosa, and occasionally in the endothelium of small vessels of the stromal tissues. In conclusion, TLR2, TLR4 and TLR9 expression may be important in the biological pathogenesis of UC. TLR alterations in the innate response system may contribute to the pathogenesis of UC. |
| publisher |
D.A. Spandidos |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353785/ |
| _version_ |
1613196659945111552 |