Alcohol Induced Alterations to the Human Fecal VOC Metabolome

Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and tox...

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Main Authors:	Couch, Robin D., Dailey, Allyson, Zaidi, Fatima, Navarro, Karl, Forsyth, Christopher B., Mutlu, Ece, Engen, Phillip A., Keshavarzian, Ali
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353727/

id	pubmed-4353727
recordtype	oai_dc
spelling	pubmed-43537272015-03-17 Alcohol Induced Alterations to the Human Fecal VOC Metabolome Couch, Robin D. Dailey, Allyson Zaidi, Fatima Navarro, Karl Forsyth, Christopher B. Mutlu, Ece Engen, Phillip A. Keshavarzian, Ali Research Article Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies. Public Library of Science 2015-03-09 /pmc/articles/PMC4353727/ /pubmed/25751150 http://dx.doi.org/10.1371/journal.pone.0119362 Text en © 2015 Couch et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Couch, Robin D. Dailey, Allyson Zaidi, Fatima Navarro, Karl Forsyth, Christopher B. Mutlu, Ece Engen, Phillip A. Keshavarzian, Ali
spellingShingle	Couch, Robin D. Dailey, Allyson Zaidi, Fatima Navarro, Karl Forsyth, Christopher B. Mutlu, Ece Engen, Phillip A. Keshavarzian, Ali Alcohol Induced Alterations to the Human Fecal VOC Metabolome
author_facet	Couch, Robin D. Dailey, Allyson Zaidi, Fatima Navarro, Karl Forsyth, Christopher B. Mutlu, Ece Engen, Phillip A. Keshavarzian, Ali
author_sort	Couch, Robin D.
title	Alcohol Induced Alterations to the Human Fecal VOC Metabolome
title_short	Alcohol Induced Alterations to the Human Fecal VOC Metabolome
title_full	Alcohol Induced Alterations to the Human Fecal VOC Metabolome
title_fullStr	Alcohol Induced Alterations to the Human Fecal VOC Metabolome
title_full_unstemmed	Alcohol Induced Alterations to the Human Fecal VOC Metabolome
title_sort	alcohol induced alterations to the human fecal voc metabolome
description	Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353727/
_version_	1613196626930696192

Alcohol Induced Alterations to the Human Fecal VOC Metabolome

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