Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool sa...
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Hindawi Publishing Corporation
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353443/ |
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pubmed-43534432015-03-23 Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer Li, Wen-han Zhang, Hao Guo, Qi Wu, Xuan-di Xu, Zi-sen Dang, Cheng-xue Xia, Peng Song, Yong-chun Research Article Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool samples were collected as healthy controls. Results. The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP). The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P < 0.001). In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P < 0.001). The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%. In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P = 0.039; P = 0.006, resp.). Conclusion. We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer. Hindawi Publishing Corporation 2015 2015-02-23 /pmc/articles/PMC4353443/ /pubmed/25802477 http://dx.doi.org/10.1155/2015/657570 Text en Copyright © 2015 Wen-han Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Li, Wen-han Zhang, Hao Guo, Qi Wu, Xuan-di Xu, Zi-sen Dang, Cheng-xue Xia, Peng Song, Yong-chun |
| spellingShingle |
Li, Wen-han Zhang, Hao Guo, Qi Wu, Xuan-di Xu, Zi-sen Dang, Cheng-xue Xia, Peng Song, Yong-chun Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| author_facet |
Li, Wen-han Zhang, Hao Guo, Qi Wu, Xuan-di Xu, Zi-sen Dang, Cheng-xue Xia, Peng Song, Yong-chun |
| author_sort |
Li, Wen-han |
| title |
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| title_short |
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| title_full |
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| title_fullStr |
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| title_full_unstemmed |
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer |
| title_sort |
detection of snca and fbn1 methylation in the stool as a biomarker for colorectal cancer |
| description |
Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool samples were collected as healthy controls. Results. The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP). The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P < 0.001). In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P < 0.001). The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%. In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P = 0.039; P = 0.006, resp.). Conclusion. We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353443/ |
| _version_ |
1613196554775035904 |