Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR

Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR

Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms rema...

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Main Authors: Tavernier, Sarah, Coenye, Tom
Format: Online
Language:English
Published: PeerJ Inc. 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349053/
id pubmed-4349053
recordtype oai_dc
spelling pubmed-43490532015-03-09 Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR Tavernier, Sarah Coenye, Tom Microbiology Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach. PeerJ Inc. 2015-02-24 /pmc/articles/PMC4349053/ /pubmed/25755923 http://dx.doi.org/10.7717/peerj.787 Text en © 2015 Tavernier and Coenye http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Tavernier, Sarah
Coenye, Tom
spellingShingle Tavernier, Sarah
Coenye, Tom
Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
author_facet Tavernier, Sarah
Coenye, Tom
author_sort Tavernier, Sarah
title Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
title_short Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
title_full Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
title_fullStr Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
title_full_unstemmed Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
title_sort quantification of pseudomonas aeruginosa in multispecies biofilms using pma-qpcr
description Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.
publisher PeerJ Inc.
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349053/
_version_ 1613194988788645888

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