Pulmonary Tuberculosis Caused by Mycobacterium bovis in China

The epidemiology of Mycobacterium bovis infection in humans in China is unknown. In this study, pulmonary tuberculosis caused by M. bovis in China was studied. A total of 4069 clinical strains isolated from sputa during the 2007–2009 nationwide surveillance of drug-resistant tuberculosis in China we...

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Main Authors:	Jiang, Guanglu, Wang, Guirong, Chen, Suting, Yu, Xia, Wang, Xiaobo, Zhao, Liping, Ma, Yifeng, Dong, Lingling, Huang, Hairong
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348663/

id	pubmed-4348663
recordtype	oai_dc
spelling	pubmed-43486632015-03-10 Pulmonary Tuberculosis Caused by Mycobacterium bovis in China Jiang, Guanglu Wang, Guirong Chen, Suting Yu, Xia Wang, Xiaobo Zhao, Liping Ma, Yifeng Dong, Lingling Huang, Hairong Article The epidemiology of Mycobacterium bovis infection in humans in China is unknown. In this study, pulmonary tuberculosis caused by M. bovis in China was studied. A total of 4069 clinical strains isolated from sputa during the 2007–2009 nationwide surveillance of drug-resistant tuberculosis in China were analyzed. M. bovis was identified by para-nitrobenzoic acid and thiophen-2-carboxylic acid hydrazide growth tests, spoligotyping and multiplex PCR amplification. In addition, a total of 1828 clinical specimens were recruited from Beijing Chest Hospital (Beijing, China) for Löwenstein-Jensen (LJ) culture, both on standard LJ medium and LJ medium containing 4.5 mg/ml(W/V) sodium pyruvate, the latter being the preferred medium for M. bovis growth. The isolates which demonstrated more vigorous on pyruvate containing medium than on standard LJ medium were then identified by multiplex PCR amplification. Only 1 isolate from the nationwide surveillance was confirmed as M. bovis-BCG. The isolate belonged to a predominant spoligotype SB0120 (ST482). In addition, no M. bovis isolate was acquired by the continuous screening step in Beijing Chest Hospital. M. bovis has a negligible contribution to pulmonary tuberculosis in China, so neither laboratory identification nor clinical treatment of M. bovis infection need be considered in routine work. Nature Publishing Group 2015-03-04 /pmc/articles/PMC4348663/ /pubmed/25736338 http://dx.doi.org/10.1038/srep08538 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jiang, Guanglu Wang, Guirong Chen, Suting Yu, Xia Wang, Xiaobo Zhao, Liping Ma, Yifeng Dong, Lingling Huang, Hairong
spellingShingle	Jiang, Guanglu Wang, Guirong Chen, Suting Yu, Xia Wang, Xiaobo Zhao, Liping Ma, Yifeng Dong, Lingling Huang, Hairong Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
author_facet	Jiang, Guanglu Wang, Guirong Chen, Suting Yu, Xia Wang, Xiaobo Zhao, Liping Ma, Yifeng Dong, Lingling Huang, Hairong
author_sort	Jiang, Guanglu
title	Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
title_short	Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
title_full	Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
title_fullStr	Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
title_full_unstemmed	Pulmonary Tuberculosis Caused by Mycobacterium bovis in China
title_sort	pulmonary tuberculosis caused by mycobacterium bovis in china
description	The epidemiology of Mycobacterium bovis infection in humans in China is unknown. In this study, pulmonary tuberculosis caused by M. bovis in China was studied. A total of 4069 clinical strains isolated from sputa during the 2007–2009 nationwide surveillance of drug-resistant tuberculosis in China were analyzed. M. bovis was identified by para-nitrobenzoic acid and thiophen-2-carboxylic acid hydrazide growth tests, spoligotyping and multiplex PCR amplification. In addition, a total of 1828 clinical specimens were recruited from Beijing Chest Hospital (Beijing, China) for Löwenstein-Jensen (LJ) culture, both on standard LJ medium and LJ medium containing 4.5 mg/ml(W/V) sodium pyruvate, the latter being the preferred medium for M. bovis growth. The isolates which demonstrated more vigorous on pyruvate containing medium than on standard LJ medium were then identified by multiplex PCR amplification. Only 1 isolate from the nationwide surveillance was confirmed as M. bovis-BCG. The isolate belonged to a predominant spoligotype SB0120 (ST482). In addition, no M. bovis isolate was acquired by the continuous screening step in Beijing Chest Hospital. M. bovis has a negligible contribution to pulmonary tuberculosis in China, so neither laboratory identification nor clinical treatment of M. bovis infection need be considered in routine work.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348663/
_version_	1613194920631205888

Pulmonary Tuberculosis Caused by Mycobacterium bovis in China

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