Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress

Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O2 (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O2 were characterized. Methane production and protein content,...

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Main Authors:	Jasso-Chávez, Ricardo, Santiago-Martínez, M. Geovanni, Lira-Silva, Elizabeth, Pineda, Erika, Zepeda-Rodríguez, Armando, Belmont-Díaz, Javier, Encalada, Rusely, Saavedra, Emma, Moreno-Sánchez, Rafael
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338226/

id	pubmed-4338226
recordtype	oai_dc
spelling	pubmed-43382262015-03-04 Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael Research Article Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O2 (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O2 were characterized. Methane production and protein content, as indicators of cell growth, did not change in air-adapted cells respect to cells cultured under anoxia (control cells). In contrast, growth and methane production significantly decreased in control cells exposed for the first time to O2. Production of reactive oxygen species was 50 times lower in air-adapted cells versus control cells, suggesting enhanced anti-oxidant mechanisms that attenuated the O2 toxicity. In this regard, (i) the transcripts and activities of superoxide dismutase, catalase and peroxidase significantly increased; and (ii) the thiol-molecules (cysteine + coenzyme M-SH + sulfide) and polyphosphate contents were respectively 2 and 5 times higher in air-adapted cells versus anaerobic-control cells. Long-term cultures (18 days) of air-adapted cells exposed to 2% O2 exhibited the ability to form biofilms. These data indicate that M. acetivorans develops multiple mechanisms to contend with O2 and the associated oxidative stress, as also suggested by genome analyses for some methanogens. Public Library of Science 2015-02-23 /pmc/articles/PMC4338226/ /pubmed/25706146 http://dx.doi.org/10.1371/journal.pone.0117331 Text en © 2015 Jasso-Chávez et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael
spellingShingle	Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
author_facet	Jasso-Chávez, Ricardo Santiago-Martínez, M. Geovanni Lira-Silva, Elizabeth Pineda, Erika Zepeda-Rodríguez, Armando Belmont-Díaz, Javier Encalada, Rusely Saavedra, Emma Moreno-Sánchez, Rafael
author_sort	Jasso-Chávez, Ricardo
title	Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
title_short	Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
title_full	Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
title_fullStr	Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
title_full_unstemmed	Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
title_sort	air-adapted methanosarcina acetivorans shows high methane production and develops resistance against oxygen stress
description	Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O2 (atmospheric) for at least 6 months to generate air-adapted cells; further, the biochemical mechanisms developed to deal with O2 were characterized. Methane production and protein content, as indicators of cell growth, did not change in air-adapted cells respect to cells cultured under anoxia (control cells). In contrast, growth and methane production significantly decreased in control cells exposed for the first time to O2. Production of reactive oxygen species was 50 times lower in air-adapted cells versus control cells, suggesting enhanced anti-oxidant mechanisms that attenuated the O2 toxicity. In this regard, (i) the transcripts and activities of superoxide dismutase, catalase and peroxidase significantly increased; and (ii) the thiol-molecules (cysteine + coenzyme M-SH + sulfide) and polyphosphate contents were respectively 2 and 5 times higher in air-adapted cells versus anaerobic-control cells. Long-term cultures (18 days) of air-adapted cells exposed to 2% O2 exhibited the ability to form biofilms. These data indicate that M. acetivorans develops multiple mechanisms to contend with O2 and the associated oxidative stress, as also suggested by genome analyses for some methanogens.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338226/
_version_	1613191753039347712

Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress

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