Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma

Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5-fluorouracil (5-FU) on BGC-823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5-FU alone...

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Main Authors:	GUO, XU-FENG, YANG, ZI-RONG, WANG, JUN, LEI, XIAO-FEI, LV, XIAO-GUANG, DONG, WEI-GUO
Format:	Online
Language:	English
Published:	D.A. Spandidos 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337718/

id	pubmed-4337718
recordtype	oai_dc
spelling	pubmed-43377182015-03-05 Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma GUO, XU-FENG YANG, ZI-RONG WANG, JUN LEI, XIAO-FEI LV, XIAO-GUANG DONG, WEI-GUO Articles Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5-fluorouracil (5-FU) on BGC-823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5-FU alone or combined on BGC-823 cells was determined using a cell counting kit 8 (CCK-8) on living cells. Apoptotic morphological features and proteins expression levels were detected by Hoechst 33258 staining, an Annexin V/propidium iodide apoptosis kit and western blot analysis, respectively. Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected. Results of the CCK-8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments. The tumor volume was 90.65% of that of the controls and the mean tumor weight was only 0.125 g at the end of the experiment in the combination group compared with the control group (0.822 g). In addition, it was determined that liver and renal toxicity did not increase in combined treatment. These findings showed that puerarin and 5-FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects. D.A. Spandidos 2015-04 2014-11-27 /pmc/articles/PMC4337718/ /pubmed/25434307 http://dx.doi.org/10.3892/mmr.2014.3016 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	GUO, XU-FENG YANG, ZI-RONG WANG, JUN LEI, XIAO-FEI LV, XIAO-GUANG DONG, WEI-GUO
spellingShingle	GUO, XU-FENG YANG, ZI-RONG WANG, JUN LEI, XIAO-FEI LV, XIAO-GUANG DONG, WEI-GUO Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
author_facet	GUO, XU-FENG YANG, ZI-RONG WANG, JUN LEI, XIAO-FEI LV, XIAO-GUANG DONG, WEI-GUO
author_sort	GUO, XU-FENG
title	Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
title_short	Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
title_full	Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
title_fullStr	Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
title_full_unstemmed	Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
title_sort	synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma
description	Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5-fluorouracil (5-FU) on BGC-823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5-FU alone or combined on BGC-823 cells was determined using a cell counting kit 8 (CCK-8) on living cells. Apoptotic morphological features and proteins expression levels were detected by Hoechst 33258 staining, an Annexin V/propidium iodide apoptosis kit and western blot analysis, respectively. Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected. Results of the CCK-8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments. The tumor volume was 90.65% of that of the controls and the mean tumor weight was only 0.125 g at the end of the experiment in the combination group compared with the control group (0.822 g). In addition, it was determined that liver and renal toxicity did not increase in combined treatment. These findings showed that puerarin and 5-FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects.
publisher	D.A. Spandidos
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337718/
_version_	1613191586034745344

Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma

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