Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates

Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated...

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Main Authors:	Contreras, Gabriela, Barahona, Salvador, Sepúlveda, Dionisia, Baeza, Marcelo, Cifuentes, Víctor, Alcaíno, Jennifer
Format:	Online
Language:	English
Published:	Springer Netherlands 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333312/

id	pubmed-4333312
recordtype	oai_dc
spelling	pubmed-43333122015-02-24 Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates Contreras, Gabriela Barahona, Salvador Sepúlveda, Dionisia Baeza, Marcelo Cifuentes, Víctor Alcaíno, Jennifer Original Paper Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated from soil samples collected at King George Island, which according to their rDNA sequence analyses, were determined to be Xanthophyllomyces dendrorhous. This yeast is of biotechnological interest, because it can synthesize astaxanthin as its main carotenoid, which is a powerful antioxidant pigment used in aquaculture. Then, the aim of this work was to characterize the ANCH isolates at their molecular and phenotypic level. The isolates did not display any differences in their rDNA and COX1 gene nucleotide sequences. However, ANCH01 produces approximately sixfold more astaxanthin than other wild type strains. Moreover, even though ANCH06 and ANCH08 produce astaxanthin, their main carotenoid was β-carotene. In contrast to other X. dendrorhous strains, the ANCH isolates did not produce mycosporines. Finally, the ANCH isolates had a higher proportion of polyunsaturated fatty acids than other wild type strains. In conclusion, the reported X. dendrorhous isolates are phenotypically different from other wild type strains, including characteristics that could make them more resistant and better able to inhabit their original habitat, which may also have biotechnological potential. Springer Netherlands 2015-02-03 2015 /pmc/articles/PMC4333312/ /pubmed/25643668 http://dx.doi.org/10.1007/s11274-015-1808-3 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Contreras, Gabriela Barahona, Salvador Sepúlveda, Dionisia Baeza, Marcelo Cifuentes, Víctor Alcaíno, Jennifer
spellingShingle	Contreras, Gabriela Barahona, Salvador Sepúlveda, Dionisia Baeza, Marcelo Cifuentes, Víctor Alcaíno, Jennifer Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
author_facet	Contreras, Gabriela Barahona, Salvador Sepúlveda, Dionisia Baeza, Marcelo Cifuentes, Víctor Alcaíno, Jennifer
author_sort	Contreras, Gabriela
title	Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
title_short	Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
title_full	Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
title_fullStr	Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
title_full_unstemmed	Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates
title_sort	identification and analysis of metabolite production with biotechnological potential in xanthophyllomyces dendrorhous isolates
description	Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated from soil samples collected at King George Island, which according to their rDNA sequence analyses, were determined to be Xanthophyllomyces dendrorhous. This yeast is of biotechnological interest, because it can synthesize astaxanthin as its main carotenoid, which is a powerful antioxidant pigment used in aquaculture. Then, the aim of this work was to characterize the ANCH isolates at their molecular and phenotypic level. The isolates did not display any differences in their rDNA and COX1 gene nucleotide sequences. However, ANCH01 produces approximately sixfold more astaxanthin than other wild type strains. Moreover, even though ANCH06 and ANCH08 produce astaxanthin, their main carotenoid was β-carotene. In contrast to other X. dendrorhous strains, the ANCH isolates did not produce mycosporines. Finally, the ANCH isolates had a higher proportion of polyunsaturated fatty acids than other wild type strains. In conclusion, the reported X. dendrorhous isolates are phenotypically different from other wild type strains, including characteristics that could make them more resistant and better able to inhabit their original habitat, which may also have biotechnological potential.
publisher	Springer Netherlands
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333312/
_version_	1613190050241052672

Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates

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