Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex

In mammalian cerebrum there exist two distinct types of interneurons expressing nitric oxide synthase (NOS). Type I neurons are large in size and exhibit heavy nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical reaction, while type II cells are small with light NADPH-d re...

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Main Authors:	Liu, Chao, Yang, Yan, Hu, Xia, Li, Jian-Ming, Zhang, Xue-Mei, Cai, Yan, Li, Zhiyuan, Yan, Xiao-Xin
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329812/

id	pubmed-4329812
recordtype	oai_dc
spelling	pubmed-43298122015-03-11 Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex Liu, Chao Yang, Yan Hu, Xia Li, Jian-Ming Zhang, Xue-Mei Cai, Yan Li, Zhiyuan Yan, Xiao-Xin Neuroscience In mammalian cerebrum there exist two distinct types of interneurons expressing nitric oxide synthase (NOS). Type I neurons are large in size and exhibit heavy nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical reaction, while type II cells are small with light NADPH-d reactivity. The time of origin of these cortical neurons relative to corticogenesis remains largely unclear among mammals. Here we explored this issue in guinea pigs using cell birth-dating and double-labeling methods. Bromodeoxyuridine (BrdU) pulse-chasing (2 doses at 50 mg/kg, 12 h apart) was given to time-pregnant mothers, followed by quantification of NADPH-d/BrdU colocalization in the parietal and temporal neocortex in offspring at postnatal day 0 (P0), P30 and P60. Type I neurons were partially colabeled with BrdU at P0, P30 and P60 following pulse-chasing at embryonic day 21 (E21), E28 and E35, varied from 2–11.3% of total population of these neurons for the three time groups. Type II neurons were partially colabeled for BrdU following pulse-chasing at E21, E28, E35 and E42 at P0 (8.6%–16.5% of total population for individual time groups). At P60, type II neurons were found to co-express BrdU (4.8–11.3% of total population for individual time groups) following pulse-chasing at E21, E28, E35, E42, E49, E56 and E60/61. These results indicate that in guinea pigs type I neurons are generated during early corticogenesis, whereas type II cells are produced over a wide prenatal time window persisting until birth. The data also suggest that type II nitrinergic neurons may undergo a period of development/differentiation, for over 1 month, before being NADPH-d reactive. Frontiers Media S.A. 2015-02-16 /pmc/articles/PMC4329812/ /pubmed/25762900 http://dx.doi.org/10.3389/fnana.2015.00011 Text en Copyright © 2015 Liu, Yang, Hu, Li, Zhang, Cai, Li and Yan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Chao Yang, Yan Hu, Xia Li, Jian-Ming Zhang, Xue-Mei Cai, Yan Li, Zhiyuan Yan, Xiao-Xin
spellingShingle	Liu, Chao Yang, Yan Hu, Xia Li, Jian-Ming Zhang, Xue-Mei Cai, Yan Li, Zhiyuan Yan, Xiao-Xin Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
author_facet	Liu, Chao Yang, Yan Hu, Xia Li, Jian-Ming Zhang, Xue-Mei Cai, Yan Li, Zhiyuan Yan, Xiao-Xin
author_sort	Liu, Chao
title	Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
title_short	Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
title_full	Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
title_fullStr	Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
title_full_unstemmed	Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
title_sort	ontogenesis of nadph-diaphorase positive neurons in guinea pig neocortex
description	In mammalian cerebrum there exist two distinct types of interneurons expressing nitric oxide synthase (NOS). Type I neurons are large in size and exhibit heavy nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical reaction, while type II cells are small with light NADPH-d reactivity. The time of origin of these cortical neurons relative to corticogenesis remains largely unclear among mammals. Here we explored this issue in guinea pigs using cell birth-dating and double-labeling methods. Bromodeoxyuridine (BrdU) pulse-chasing (2 doses at 50 mg/kg, 12 h apart) was given to time-pregnant mothers, followed by quantification of NADPH-d/BrdU colocalization in the parietal and temporal neocortex in offspring at postnatal day 0 (P0), P30 and P60. Type I neurons were partially colabeled with BrdU at P0, P30 and P60 following pulse-chasing at embryonic day 21 (E21), E28 and E35, varied from 2–11.3% of total population of these neurons for the three time groups. Type II neurons were partially colabeled for BrdU following pulse-chasing at E21, E28, E35 and E42 at P0 (8.6%–16.5% of total population for individual time groups). At P60, type II neurons were found to co-express BrdU (4.8–11.3% of total population for individual time groups) following pulse-chasing at E21, E28, E35, E42, E49, E56 and E60/61. These results indicate that in guinea pigs type I neurons are generated during early corticogenesis, whereas type II cells are produced over a wide prenatal time window persisting until birth. The data also suggest that type II nitrinergic neurons may undergo a period of development/differentiation, for over 1 month, before being NADPH-d reactive.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329812/
_version_	1613188817931468800

Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex

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