A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples

A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples

NMR spectroscopy is a commonly used technique for metabolite analyses. Due to the observed macroscopic magnetic susceptibility in biological tissues, current NMR acquisitions in measurements of biological tissues are generally performed on tissue extracts using liquid NMR or on tissues using magic-a...

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Main Authors: Huang, Yuqing, Zhang, Zhiyong, Chen, Hao, Feng, Jianghua, Cai, Shuhui, Chen, Zhong
Format: Online
Language:English
Published: Nature Publishing Group 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323641/
id pubmed-4323641
recordtype oai_dc
spelling pubmed-43236412015-02-20 A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples Huang, Yuqing Zhang, Zhiyong Chen, Hao Feng, Jianghua Cai, Shuhui Chen, Zhong Article NMR spectroscopy is a commonly used technique for metabolite analyses. Due to the observed macroscopic magnetic susceptibility in biological tissues, current NMR acquisitions in measurements of biological tissues are generally performed on tissue extracts using liquid NMR or on tissues using magic-angle spinning techniques. In this study, we propose an NMR method to achieve high-resolution J-resolved information for metabolite analyses directly from intact biological samples. A dramatic improvement in spectral resolution is evident in our contrastive demonstrations on a sample of pig brain tissue. Metabolite analyses for a postmortem fish from fresh to decayed statuses are presented to further reveal the capability of the proposed method. This method is a previously-unreported high-resolution 2D J-resolved spectroscopy for biological applications without specialised hardware requirements or complicated sample pretreatments. It provides a significant contribution to metabolite analyses of biological samples, and may be potentially applicable to in vivo samples. Furthermore, this method also can be applied to measurements of semisolid and viscous samples. Nature Publishing Group 2015-02-11 /pmc/articles/PMC4323641/ /pubmed/25670027 http://dx.doi.org/10.1038/srep08390 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Huang, Yuqing
Zhang, Zhiyong
Chen, Hao
Feng, Jianghua
Cai, Shuhui
Chen, Zhong
spellingShingle Huang, Yuqing
Zhang, Zhiyong
Chen, Hao
Feng, Jianghua
Cai, Shuhui
Chen, Zhong
A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
author_facet Huang, Yuqing
Zhang, Zhiyong
Chen, Hao
Feng, Jianghua
Cai, Shuhui
Chen, Zhong
author_sort Huang, Yuqing
title A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
title_short A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
title_full A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
title_fullStr A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
title_full_unstemmed A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples
title_sort high-resolution 2d j-resolved nmr detection technique for metabolite analyses of biological samples
description NMR spectroscopy is a commonly used technique for metabolite analyses. Due to the observed macroscopic magnetic susceptibility in biological tissues, current NMR acquisitions in measurements of biological tissues are generally performed on tissue extracts using liquid NMR or on tissues using magic-angle spinning techniques. In this study, we propose an NMR method to achieve high-resolution J-resolved information for metabolite analyses directly from intact biological samples. A dramatic improvement in spectral resolution is evident in our contrastive demonstrations on a sample of pig brain tissue. Metabolite analyses for a postmortem fish from fresh to decayed statuses are presented to further reveal the capability of the proposed method. This method is a previously-unreported high-resolution 2D J-resolved spectroscopy for biological applications without specialised hardware requirements or complicated sample pretreatments. It provides a significant contribution to metabolite analyses of biological samples, and may be potentially applicable to in vivo samples. Furthermore, this method also can be applied to measurements of semisolid and viscous samples.
publisher Nature Publishing Group
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323641/
_version_ 1613186516144619520

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