A repository of assays to quantify 10,000 human proteins by SWATH-MS
Mass spectrometry is the method of choice for deep and reliable exploration of the (human) proteome. Targeted mass spectrometry reliably detects and quantifies pre-determined sets of proteins in a complex biological matrix and is used in studies that rely on the quantitatively accurate and reproduci...
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| Format: | Online |
| Language: | English |
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Nature Publishing Group
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322573/ |
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pubmed-4322573 |
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pubmed-43225732015-05-14 A repository of assays to quantify 10,000 human proteins by SWATH-MS Rosenberger, George Koh, Ching Chiek Guo, Tiannan Röst, Hannes L. Kouvonen, Petri Collins, Ben C. Heusel, Moritz Liu, Yansheng Caron, Etienne Vichalkovski, Anton Faini, Marco Schubert, Olga T. Faridi, Pouya Ebhardt, H. Alexander Matondo, Mariette Lam, Henry Bader, Samuel L. Campbell, David S. Deutsch, Eric W. Moritz, Robert L. Tate, Stephen Aebersold, Ruedi Data Descriptor Mass spectrometry is the method of choice for deep and reliable exploration of the (human) proteome. Targeted mass spectrometry reliably detects and quantifies pre-determined sets of proteins in a complex biological matrix and is used in studies that rely on the quantitatively accurate and reproducible measurement of proteins across multiple samples. It requires the one-time, a priori generation of a specific measurement assay for each targeted protein. SWATH-MS is a mass spectrometric method that combines data-independent acquisition (DIA) and targeted data analysis and vastly extends the throughput of proteins that can be targeted in a sample compared to selected reaction monitoring (SRM). Here we present a compendium of highly specific assays covering more than 10,000 human proteins and enabling their targeted analysis in SWATH-MS datasets acquired from research or clinical specimens. This resource supports the confident detection and quantification of 50.9% of all human proteins annotated by UniProtKB/Swiss-Prot and is therefore expected to find wide application in basic and clinical research. Data are available via ProteomeXchange (PXD000953-954) and SWATHAtlas (SAL00016-35). Nature Publishing Group 2014-09-16 /pmc/articles/PMC4322573/ /pubmed/25977788 http://dx.doi.org/10.1038/sdata.2014.31 Text en Copyright © 2014, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Rosenberger, George Koh, Ching Chiek Guo, Tiannan Röst, Hannes L. Kouvonen, Petri Collins, Ben C. Heusel, Moritz Liu, Yansheng Caron, Etienne Vichalkovski, Anton Faini, Marco Schubert, Olga T. Faridi, Pouya Ebhardt, H. Alexander Matondo, Mariette Lam, Henry Bader, Samuel L. Campbell, David S. Deutsch, Eric W. Moritz, Robert L. Tate, Stephen Aebersold, Ruedi |
| spellingShingle |
Rosenberger, George Koh, Ching Chiek Guo, Tiannan Röst, Hannes L. Kouvonen, Petri Collins, Ben C. Heusel, Moritz Liu, Yansheng Caron, Etienne Vichalkovski, Anton Faini, Marco Schubert, Olga T. Faridi, Pouya Ebhardt, H. Alexander Matondo, Mariette Lam, Henry Bader, Samuel L. Campbell, David S. Deutsch, Eric W. Moritz, Robert L. Tate, Stephen Aebersold, Ruedi A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| author_facet |
Rosenberger, George Koh, Ching Chiek Guo, Tiannan Röst, Hannes L. Kouvonen, Petri Collins, Ben C. Heusel, Moritz Liu, Yansheng Caron, Etienne Vichalkovski, Anton Faini, Marco Schubert, Olga T. Faridi, Pouya Ebhardt, H. Alexander Matondo, Mariette Lam, Henry Bader, Samuel L. Campbell, David S. Deutsch, Eric W. Moritz, Robert L. Tate, Stephen Aebersold, Ruedi |
| author_sort |
Rosenberger, George |
| title |
A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| title_short |
A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| title_full |
A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| title_fullStr |
A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| title_full_unstemmed |
A repository of assays to quantify 10,000 human proteins by SWATH-MS |
| title_sort |
repository of assays to quantify 10,000 human proteins by swath-ms |
| description |
Mass spectrometry is the method of choice for deep and reliable exploration of the (human) proteome. Targeted mass spectrometry reliably detects and quantifies pre-determined sets of proteins in a complex biological matrix and is used in studies that rely on the quantitatively accurate and reproducible measurement of proteins across multiple samples. It requires the one-time, a priori generation of a specific measurement assay for each targeted protein. SWATH-MS is a mass spectrometric method that combines data-independent acquisition (DIA) and targeted data analysis and vastly extends the throughput of proteins that can be targeted in a sample compared to selected reaction monitoring (SRM). Here we present a compendium of highly specific assays covering more than 10,000 human proteins and enabling their targeted analysis in SWATH-MS datasets acquired from research or clinical specimens. This resource supports the confident detection and quantification of 50.9% of all human proteins annotated by UniProtKB/Swiss-Prot and is therefore expected to find wide application in basic and clinical research. Data are available via ProteomeXchange (PXD000953-954) and SWATHAtlas (SAL00016-35). |
| publisher |
Nature Publishing Group |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322573/ |
| _version_ |
1613186102661742592 |