Multicopy integration of mini-Tn7 transposons into selected chromosomal sites of a Salmonella vaccine strain

Chromosomal integration of expression modules for transgenes is an important aspect for the development of novel Salmonella vectors. Mini-Tn7 transposons have been used for the insertion of one such module into the chromosomal site attTn7, present only once in most Gram-negative bacteria. However, i...

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Bibliographic Details
Main Authors: Roos, Karen, Werner, Esther, Loessner, Holger
Format: Online
Language:English
Published: BlackWell Publishing Ltd 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321384/