Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignment...

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Bibliographic Details
Main Authors: Bacci, Giovanni, Bani, Alessia, Bazzicalupo, Marco, Ceccherini, Maria Teresa, Galardini, Marco, Nannipieri, Paolo, Pietramellara, Giacomo, Mengoni, Alessio
Format: Online
Language:English
Published: Ivyspring International Publisher 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316179/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316179/

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