OsSDS is essential for DSB formation in rice meiosis

SDS is a meiosis specific cyclin-like protein and required for DMC1 mediated double-strand break (DSB) repairing in Arabidopsis. Here, we found its rice homolog, OsSDS, is essential for meiotic DSB formation. The Ossds mutant is normal in vegetative growth but both male and female gametes are inviab...

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Main Authors:	Wu, Zhigang, Ji, Jianhui, Tang, Ding, Wang, Hongjun, Shen, Yi, Shi, Wenqing, Li, Yafei, Tan, Xuelin, Cheng, Zhukuan, Luo, Qiong
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315026/

id	pubmed-4315026
recordtype	oai_dc
spelling	pubmed-43150262015-02-17 OsSDS is essential for DSB formation in rice meiosis Wu, Zhigang Ji, Jianhui Tang, Ding Wang, Hongjun Shen, Yi Shi, Wenqing Li, Yafei Tan, Xuelin Cheng, Zhukuan Luo, Qiong Plant Science SDS is a meiosis specific cyclin-like protein and required for DMC1 mediated double-strand break (DSB) repairing in Arabidopsis. Here, we found its rice homolog, OsSDS, is essential for meiotic DSB formation. The Ossds mutant is normal in vegetative growth but both male and female gametes are inviable. The Ossds meiocytes exhibit severe defects in homologous pairing and synapsis. No γH2AX immunosignals in Ossds meiocytes together with the suppression of chromosome fragmentation in Ossds-1 Osrad51c, both provide strong evidences that OsSDS is essential for meiotic DSB formation. Immunostaining investigations revealed that meiotic chromosome axes are normally formed but both SC installation and localization of recombination elements are failed in Ossds. We suspected that this cyclin protein has been differentiated pretty much between monocots and dicots on its function in meiosis. Frontiers Media S.A. 2015-02-03 /pmc/articles/PMC4315026/ /pubmed/25691887 http://dx.doi.org/10.3389/fpls.2015.00021 Text en Copyright © 2015 Wu, Ji, Tang, Wang, Shen, Shi, Li, Tan, Cheng and Luo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wu, Zhigang Ji, Jianhui Tang, Ding Wang, Hongjun Shen, Yi Shi, Wenqing Li, Yafei Tan, Xuelin Cheng, Zhukuan Luo, Qiong
spellingShingle	Wu, Zhigang Ji, Jianhui Tang, Ding Wang, Hongjun Shen, Yi Shi, Wenqing Li, Yafei Tan, Xuelin Cheng, Zhukuan Luo, Qiong OsSDS is essential for DSB formation in rice meiosis
author_facet	Wu, Zhigang Ji, Jianhui Tang, Ding Wang, Hongjun Shen, Yi Shi, Wenqing Li, Yafei Tan, Xuelin Cheng, Zhukuan Luo, Qiong
author_sort	Wu, Zhigang
title	OsSDS is essential for DSB formation in rice meiosis
title_short	OsSDS is essential for DSB formation in rice meiosis
title_full	OsSDS is essential for DSB formation in rice meiosis
title_fullStr	OsSDS is essential for DSB formation in rice meiosis
title_full_unstemmed	OsSDS is essential for DSB formation in rice meiosis
title_sort	ossds is essential for dsb formation in rice meiosis
description	SDS is a meiosis specific cyclin-like protein and required for DMC1 mediated double-strand break (DSB) repairing in Arabidopsis. Here, we found its rice homolog, OsSDS, is essential for meiotic DSB formation. The Ossds mutant is normal in vegetative growth but both male and female gametes are inviable. The Ossds meiocytes exhibit severe defects in homologous pairing and synapsis. No γH2AX immunosignals in Ossds meiocytes together with the suppression of chromosome fragmentation in Ossds-1 Osrad51c, both provide strong evidences that OsSDS is essential for meiotic DSB formation. Immunostaining investigations revealed that meiotic chromosome axes are normally formed but both SC installation and localization of recombination elements are failed in Ossds. We suspected that this cyclin protein has been differentiated pretty much between monocots and dicots on its function in meiosis.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315026/
_version_	1613183601076076544

OsSDS is essential for DSB formation in rice meiosis

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