High Prevalence of BlaCTX-M Group Genes in Aeromonas dhakensis Isolated from Aquaculture Fish Species in South Korea
The prevalence of resistant genes against β-lactams in 119 Aeromonas strains was determined. A large number (99.2%) of the present fish strains were resistant to one or more β- lactams including ceftiofur, amoxicillin-clavulanic acid, ampicillin, piperacillin and cefpodoxime. Among antibiotic resist...
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The Japanese Society of Veterinary Science
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300373/ |
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pubmed-4300373 |
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pubmed-43003732015-02-06 High Prevalence of BlaCTX-M Group Genes in Aeromonas dhakensis Isolated from Aquaculture Fish Species in South Korea YI, Seung-Won CHUNG, Tae-Ho JOH, Seong-Joon PARK, Chul PARK, Byoung-Yong SHIN, Gee-Wook Bacteriology The prevalence of resistant genes against β-lactams in 119 Aeromonas strains was determined. A large number (99.2%) of the present fish strains were resistant to one or more β- lactams including ceftiofur, amoxicillin-clavulanic acid, ampicillin, piperacillin and cefpodoxime. Among antibiotic resistance phenotypes, the simultaneous resistance to all β-lactams occurred in 25.2% (n=30) of all strains, which consisted of 18 strains of A. dhakensis, 8 strains of A. caviae, 2 strains of A. hydrophila and only one strain of A. veronii. For exploring genetic background of the antibiotic resistances, multiple PCR assays were subjected to detect β-lactamase-encoding genes, blaTEM, blaOXA-B and blaCTX-M. In the results, the blaTEM-1 gene was harbored in all strains, whereas only 3 strains harbored blaOXA gene. In the case of blaCTX-M gene, the gene was detected in 21.0% (25 out of 119) of all strains, which countered with 80% (20 out of 25) of A. dhakensis, 8% (2 out of 25) of A. caviae and 12% (3 out of 25) of A. hydrophila. In addition, most of the blaCTX-M positive strains showed simultaneous resistance to all β-lactams (18 out of 30 strains). In sequence analysis for blaCTX-M genes detected, they were CTX-M group 1-encoding genes including blaCTX-M-33 from 3 eel strains of A. dhakensis. Therefore, A. dhakensis obtained from cultured fish could represent a reservoir for spreading genes encoding CTX-M group 1 enzymes and hence should be carefully monitored, especially for its potential risk to public health. The Japanese Society of Veterinary Science 2014-09-03 2014-12 /pmc/articles/PMC4300373/ /pubmed/25649940 http://dx.doi.org/10.1292/jvms.14-0274 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
YI, Seung-Won CHUNG, Tae-Ho JOH, Seong-Joon PARK, Chul PARK, Byoung-Yong SHIN, Gee-Wook |
| spellingShingle |
YI, Seung-Won CHUNG, Tae-Ho JOH, Seong-Joon PARK, Chul PARK, Byoung-Yong SHIN, Gee-Wook High Prevalence of BlaCTX-M Group Genes in Aeromonas dhakensis Isolated from Aquaculture Fish Species in South Korea |
| author_facet |
YI, Seung-Won CHUNG, Tae-Ho JOH, Seong-Joon PARK, Chul PARK, Byoung-Yong SHIN, Gee-Wook |
| author_sort |
YI, Seung-Won |
| title |
High Prevalence of BlaCTX-M Group Genes in
Aeromonas dhakensis Isolated from Aquaculture Fish Species in South
Korea |
| title_short |
High Prevalence of BlaCTX-M Group Genes in
Aeromonas dhakensis Isolated from Aquaculture Fish Species in South
Korea |
| title_full |
High Prevalence of BlaCTX-M Group Genes in
Aeromonas dhakensis Isolated from Aquaculture Fish Species in South
Korea |
| title_fullStr |
High Prevalence of BlaCTX-M Group Genes in
Aeromonas dhakensis Isolated from Aquaculture Fish Species in South
Korea |
| title_full_unstemmed |
High Prevalence of BlaCTX-M Group Genes in
Aeromonas dhakensis Isolated from Aquaculture Fish Species in South
Korea |
| title_sort |
high prevalence of blactx-m group genes in
aeromonas dhakensis isolated from aquaculture fish species in south
korea |
| description |
The prevalence of resistant
genes against β-lactams in 119 Aeromonas strains was determined. A large
number (99.2%) of the present fish strains were resistant to one or more β- lactams
including ceftiofur, amoxicillin-clavulanic acid, ampicillin, piperacillin and
cefpodoxime. Among antibiotic resistance phenotypes, the simultaneous resistance to all
β-lactams occurred in 25.2% (n=30) of all strains, which consisted of 18 strains of
A. dhakensis, 8 strains of A. caviae, 2 strains of
A. hydrophila and only one strain of A. veronii. For
exploring genetic background of the antibiotic resistances, multiple PCR assays were
subjected to detect β-lactamase-encoding genes, blaTEM,
blaOXA-B and blaCTX-M. In the
results, the blaTEM-1 gene was harbored in all strains,
whereas only 3 strains harbored blaOXA gene. In the case of
blaCTX-M gene, the gene was detected in 21.0% (25 out of
119) of all strains, which countered with 80% (20 out of 25) of A.
dhakensis, 8% (2 out of 25) of A. caviae and 12% (3 out of 25)
of A. hydrophila. In addition, most of the
blaCTX-M positive strains showed simultaneous resistance to
all β-lactams (18 out of 30 strains). In sequence analysis for
blaCTX-M genes detected, they were CTX-M group 1-encoding
genes including blaCTX-M-33 from 3 eel strains of A.
dhakensis. Therefore, A. dhakensis obtained from cultured fish
could represent a reservoir for spreading genes encoding CTX-M group 1 enzymes and hence
should be carefully monitored, especially for its potential risk to public health. |
| publisher |
The Japanese Society of Veterinary Science |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300373/ |
| _version_ |
1613178617110462464 |