Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)

Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)

Histone acetylation and deacetylation at the N-terminus of histone tails play crucial roles in the regulation of eukaryotic gene activity. Histone acetylation and deacetylation are catalyzed by histone acetyltransferases and histone deacetylases (HDACs), respectively. A growing number of studies hav...

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Main Authors: Zhao, Linmao, Lu, Jingxia, Zhang, Jianxia, Wu, Pei-Ying, Yang, Songguang, Wu, Keqiang
Format: Online
Language:English
Published: Frontiers Media S.A. 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285013/
id pubmed-4285013
recordtype oai_dc
spelling pubmed-42850132015-01-21 Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum) Zhao, Linmao Lu, Jingxia Zhang, Jianxia Wu, Pei-Ying Yang, Songguang Wu, Keqiang Plant Science Histone acetylation and deacetylation at the N-terminus of histone tails play crucial roles in the regulation of eukaryotic gene activity. Histone acetylation and deacetylation are catalyzed by histone acetyltransferases and histone deacetylases (HDACs), respectively. A growing number of studies have demonstrated the importance of histone deacetylation/acetylation on genome stability, transcriptional regulation, development and response to stress in Arabidopsis. However, the biological functions of HDACs in tomato have not been investigated previously. Fifteen HDACs identified from tomato (Solanum lycopersicum) can be grouped into RPD3/HDA1, SIR2 and HD2 families based on phylogenetic analysis. Meanwhile, 10 members of the RPD3/HDA1 family can be further subdivided into four groups, namely Class I, Class II, Class III, and Class IV. High similarities of protein sequences and conserved domains were identified among SlHDACs and their homologs in Arabidopsis. Most SlHDACs were expressed in all tissues examined with different transcript abundance. Transient expression in Arabidopsis protoplasts showed that SlHDA8, SlHDA1, SlHDA5, SlSRT1 and members of the HD2 family were localized to the nucleus, whereas SlHDA3 and SlHDA4 were localized in both the cytoplasm and nucleus. The difference in the expression patterns and subcellular localization of SlHDACs suggest that they may play distinct functions in tomato. Furthermore, we found that three members of the RPD3/HDA1 family, SlHDA1, SIHDA3 and SlHDA4, interacted with TAG1 (TOMATO AGAMOUS1) and TM29 (TOMATO MADS BOX29), two MADS-box proteins associated with tomato reproductive development, indicating that these HDACs may be involved in gene regulation in reproductive development. Frontiers Media S.A. 2015-01-06 /pmc/articles/PMC4285013/ /pubmed/25610445 http://dx.doi.org/10.3389/fpls.2014.00760 Text en Copyright © 2015 Zhao, Lu, Zhang, Wu, Yang and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Zhao, Linmao
Lu, Jingxia
Zhang, Jianxia
Wu, Pei-Ying
Yang, Songguang
Wu, Keqiang
spellingShingle Zhao, Linmao
Lu, Jingxia
Zhang, Jianxia
Wu, Pei-Ying
Yang, Songguang
Wu, Keqiang
Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
author_facet Zhao, Linmao
Lu, Jingxia
Zhang, Jianxia
Wu, Pei-Ying
Yang, Songguang
Wu, Keqiang
author_sort Zhao, Linmao
title Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
title_short Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
title_full Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
title_fullStr Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
title_full_unstemmed Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum)
title_sort identification and characterization of histone deacetylases in tomato (solanum lycopersicum)
description Histone acetylation and deacetylation at the N-terminus of histone tails play crucial roles in the regulation of eukaryotic gene activity. Histone acetylation and deacetylation are catalyzed by histone acetyltransferases and histone deacetylases (HDACs), respectively. A growing number of studies have demonstrated the importance of histone deacetylation/acetylation on genome stability, transcriptional regulation, development and response to stress in Arabidopsis. However, the biological functions of HDACs in tomato have not been investigated previously. Fifteen HDACs identified from tomato (Solanum lycopersicum) can be grouped into RPD3/HDA1, SIR2 and HD2 families based on phylogenetic analysis. Meanwhile, 10 members of the RPD3/HDA1 family can be further subdivided into four groups, namely Class I, Class II, Class III, and Class IV. High similarities of protein sequences and conserved domains were identified among SlHDACs and their homologs in Arabidopsis. Most SlHDACs were expressed in all tissues examined with different transcript abundance. Transient expression in Arabidopsis protoplasts showed that SlHDA8, SlHDA1, SlHDA5, SlSRT1 and members of the HD2 family were localized to the nucleus, whereas SlHDA3 and SlHDA4 were localized in both the cytoplasm and nucleus. The difference in the expression patterns and subcellular localization of SlHDACs suggest that they may play distinct functions in tomato. Furthermore, we found that three members of the RPD3/HDA1 family, SlHDA1, SIHDA3 and SlHDA4, interacted with TAG1 (TOMATO AGAMOUS1) and TM29 (TOMATO MADS BOX29), two MADS-box proteins associated with tomato reproductive development, indicating that these HDACs may be involved in gene regulation in reproductive development.
publisher Frontiers Media S.A.
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285013/
_version_ 1613173627092467712

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