Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes

Hyaluronidase is generally used to remove cumulus cells from mouse oocytes before oocyte cryopreservation, intracytoplasmic sperm injection or DNA injection. In general, use of cumulus-free mouse oocytes decreases in vitro fertilizing ability compared with cumulus-surrounded oocytes. The effect of...

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Bibliographic Details
Main Authors: ISHIZUKA, Yuta, TAKEO, Toru, NAKAO, Satohiro, YOSHIMOTO, Hidetaka, HIROSE, Yumiko, SAKAI, Yuki, HORIKOSHI, Yuka, TAKEUJI, Shiori, TSUCHIYAMA, Shuuji, NAKAGATA, Naomi
Format: Online
Language:English
Published: The Society for Reproduction and Development 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284320/