Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens

DNA barcoding is an efficient method to identify specimens and to detect undescribed/cryptic species. Sanger sequencing of individual specimens is the standard approach in generating large-scale DNA barcode libraries and identifying unknowns. However, the Sanger sequencing technology is, in some res...

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Bibliographic Details
Main Authors: Shokralla, Shadi, Gibson, Joel F, Nikbakht, Hamid, Janzen, Daniel H, Hallwachs, Winnie, Hajibabaei, Mehrdad
Format: Online
Language:English
Published: BlackWell Publishing Ltd 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276293/